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anti-SUMF1 antibody :: Goat SUMF1 Polyclonal Antibody

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Catalog # MBS421893
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

SUMF1, Polyclonal Antibody

Full Product Name

Goat anti-SUMF1 Antibody

Product Synonym Names
SUMF1; sulfatase modifying factor 1; AAPA3037; FGE; MGC131853; MGC150436; C-alpha-formylglycine-generating enzyme; OTTHUMP00000115300; SUMF1 antibody; sulfatase modifying factor 1 antibody; AAPA3037 antibody; FGE antibody; MGC131853 antibody; MGC150436 antibody; C-alpha-formylglycine-generating enzyme antibody; OTTHUMP00000115300 antibody
Antibody/Peptide Pairs
SUMF1 peptide (MBS426423) is used for blocking the activity of SUMF1 antibody (MBS421893)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
ETLNPKGPPS GKDR
OMIM
272200
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-ETLNPKGPPSGKDR, from the internal region of the protein sequence according to NP_877437.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SUMF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SUMF1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SUMF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2). Recommended concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-SUMF1 antibody
(1ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SUMF1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SUMF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,386 Da
NCBI Official Full Name
sulfatase-modifying factor 1 isoform 1
NCBI Official Synonym Full Names
sulfatase modifying factor 1
NCBI Official Symbol
SUMF1  [Similar Products]
NCBI Official Synonym Symbols
FGE; UNQ3037; AAPA3037
  [Similar Products]
NCBI Protein Information
sulfatase-modifying factor 1
UniProt Protein Name
Sulfatase-modifying factor 1
UniProt Synonym Protein Names
C-alpha-formylglycine-generating enzyme 1
UniProt Gene Name
SUMF1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
SUMF1_HUMAN
NCBI Summary for SUMF1
This gene encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Comments for SUMF1
SUMF1: Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE. Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD). MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive. Belongs to the sulfatase-modifying factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Endoplasmic reticulum; EC 1.8.99.-; Oxidoreductase; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p26.1

Cellular Component: endoplasmic reticulum lumen

Molecular Function: metal ion binding; oxidoreductase activity; protein homodimerization activity

Biological Process: glycosphingolipid metabolic process; post-translational protein modification

Disease: Multiple Sulfatase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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