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anti-WRNIP1 antibody :: Goat WHIP/WRNIP1 Polyclonal Antibody

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Catalog # MBS420165
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

WHIP/WRNIP1, Polyclonal Antibody

Also Known As

Goat anti-WHIP/WRNIP1 Antibody

Product Synonym Names
WHIP; Werner helicase interacting protein; FLJ22526; putative helicase RUVBL; Werner helicase interacting protein 1; RP11-420G6.2; bA420G6.2; WHIP antibody; Werner helicase interacting protein antibody; FLJ22526 antibody; putative helicase RUVBL antibody; Werner helicase interacting protein 1 antibody; RP11-420G6.2 antibody; bA420G6.2 antibody; WHIP / WRNIP1
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
665
Immunogen Sequence
EELRGVDFFK QRRC
OMIM
608196
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence EELRGVDFFKQRRC, from the C Terminus of the protein sequence according to NP_064520.2; NP_569079.1.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-WRNIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-WRNIP1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-WRNIP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6ug/ml.

Western Blot (WB) of anti-WRNIP1 antibody
(1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-WRNIP1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-WRNIP1 antibody
(4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
anti-WRNIP1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for WRNIP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_569079.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,339 Da
NCBI Official Full Name
ATPase WRNIP1 isoform 1
NCBI Official Synonym Full Names
Werner helicase interacting protein 1
NCBI Official Symbol
WRNIP1  [Similar Products]
NCBI Official Synonym Symbols
WHIP; bA420G6.2
  [Similar Products]
NCBI Protein Information
ATPase WRNIP1
UniProt Protein Name
ATPase WRNIP1
UniProt Synonym Protein Names
Werner helicase-interacting protein 1
Protein Family
UniProt Gene Name
WRNIP1  [Similar Products]
UniProt Entry Name
WRIP1_HUMAN
NCBI Summary for WRNIP1
Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]
UniProt Comments for WRNIP1
WRNIP1: a modulator for initiation or restart of DNA polymerase delta-mediated DNA synthesis. Its ATPase activity may sense DNA ends and regulate the extent of polymerase stimulation.

Protein type: Helicase; EC 3.6.1.3

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: membrane; nuclear chromosome, telomeric region; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; ATPase activity; DNA binding; identical protein binding; metal ion binding; protein binding

Biological Process: DNA replication; DNA synthesis during DNA repair; regulation of DNA replication initiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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