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anti-CHAT antibody :: Goat Choline acetyltransferase Polyclonal Antibody

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Catalog # MBS420146
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Choline acetyltransferase (CHAT), Polyclonal Antibody

Also Known As

Goat anti-Choline acetyltransferase Antibody

Product Synonym Names
CHAT; choline acetyltransferase; CMS1A; CMS1A2; acetyl CoA: choline O-acetyltransferase; choline acetyltransferase antibody; CHAT antibody; CMS1A antibody; CMS1A2 antibody; acetyl CoA: choline O-acetyltransferase antibody; Choline acetyltransferase; Choline acetyltransferase
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
630
Immunogen Sequence
CKEKATRPSQ GHQP
OMIM
118490
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence according to NP_066264.3; NP_065574.3; NP_001136405.1.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CHAT antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CHAT antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0. 03-0.3ug/ml.

Western Blot (WB) of anti-CHAT antibody
(0. 03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CHAT antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_065574.3; NP_001136405.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70,394 Da
NCBI Official Full Name
choline O-acetyltransferase isoform 1
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
CMS6; CMS1A; CMS1A2; CHOACTASE
  [Similar Products]
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
UniProt Gene Name
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase  [Similar Products]
UniProt Entry Name
CLAT_HUMAN
NCBI Summary for CHAT
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
UniProt Comments for CHAT
CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: EC 2.3.1.6; Acetyltransferase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cytoplasm; cytosol; neuron projection; nucleus

Molecular Function: choline binding; choline O-acetyltransferase activity

Biological Process: acetylcholine biosynthetic process; antral ovarian follicle growth; memory; neurotransmitter secretion; phosphatidylcholine biosynthetic process; response to drug; response to ethanol; response to hypoxia; response to nutrient

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-CHAT antibody
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