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anti-ROBO3 antibody :: Goat ROBO3/RIG1 Polyclonal Antibody

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Catalog # MBS420646
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ROBO3/RIG1, Polyclonal Antibody

Also Known As

Goat anti-ROBO3/RIG1 (internal) Antibody

Product Synonym Names
Goat Anti-ROBO3 (internal) Antibody; ROBO3; RIG1; roundabout, axon guidance receptor, homolog 3 (Drosophila); FLJ21044; HGPPS; RBIG1; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3; ROBO3 antibody; RIG1 antibody; roundabout; axon guidance receptor; homolog 3 (Drosophila) antibody; FLJ21044 antibody; HGPPS antibody; RBIG1 antibody; retinoblastoma inhibiting gene 1 antibody; roundabout; axon guidance receptor; homolog 3 antibody; ROBO3 / RIG1 (internal)
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1386
Immunogen Sequence
RRKQRKELSH YT
OMIM
607313
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-RRKQRKELSHYT, from the internal region of the protein sequence according to NP_071765.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ROBO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ROBO3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ROBO3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 150kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 148kDa according to NP_071765.2). Recommended concentration: 0.5-1ug/ml.

Western Blot (WB) of anti-ROBO3 antibody
(0.5ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ROBO3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ROBO3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
110,676 Da
NCBI Official Full Name
roundabout homolog 3
NCBI Official Synonym Full Names
roundabout guidance receptor 3
NCBI Official Symbol
ROBO3  [Similar Products]
NCBI Official Synonym Symbols
HGPS; RIG1; HGPPS; RBIG1
  [Similar Products]
NCBI Protein Information
roundabout homolog 3
UniProt Protein Name
Roundabout homolog 3
UniProt Synonym Protein Names
Roundabout-like protein 3
Protein Family
UniProt Gene Name
ROBO3  [Similar Products]
UniProt Entry Name
ROBO3_HUMAN
NCBI Summary for ROBO3
This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]
UniProt Comments for ROBO3
ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: axon; integral to membrane

Biological Process: axon guidance; axon midline choice point recognition

Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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