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anti-DKK1 antibody :: Goat DKK1 Polyclonal Antibody

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Catalog # MBS421427
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

DKK1, Polyclonal Antibody

Full Product Name

Goat anti-DKK1 Antibody

Product Synonym Names
DKK1; SK; DKK-1; dickkopf homolog 1 (Xenopus laevis); dickkopf-1 like; dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1; dickkopf related protein-1; DKK1 antibody; SK antibody; DKK-1 antibody; dickkopf homolog 1 (Xenopus laevis) antibody; dickkopf-1 like antibody; dickkopf (Xenopus laevis) homolog 1 antibody; dickkopf homolog 1 antibody; dickkopf related protein-1 antibody
Antibody/Peptide Pairs
DKK1 peptide (MBS426796) is used for blocking the activity of DKK1 antibody (MBS421427)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
CDHHQASNSS RLHT
OMIM
605189
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-DKK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-DKK1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-DKK1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 38kDa band observed in Human Spleen ysates (calculated MW of 28.7kDa according to NP_036374.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fedi et al, J Biol Chem.1999 Jul 2;274(27): 19465-72; PMID: 10383463). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-DKK1 antibody
(1ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-DKK1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DKK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,672 Da
NCBI Official Full Name
dickkopf-related protein 1
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
SK; DKK-1
  [Similar Products]
NCBI Protein Information
dickkopf-related protein 1
UniProt Protein Name
Dickkopf-related protein 1
UniProt Synonym Protein Names
SK
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Dickkopf-1; Dkk-1; hDkk-1  [Similar Products]
UniProt Entry Name
DKK1_HUMAN
NCBI Summary for DKK1
This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]
UniProt Comments for DKK1
DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane

Molecular Function: growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity

Biological Process: cellular morphogenesis during differentiation; embryonic limb morphogenesis; endoderm formation; forebrain development; hair follicle development; mesoderm formation; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of skeletal muscle development; negative regulation of synaptogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; regulation of endodermal cell fate specification; regulation of receptor internalization; regulation of synaptic transmission, glutamatergic; response to retinoic acid
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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