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anti-CYP7B1 antibody :: Goat CYP7B1 Polyclonal Antibody

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Catalog # MBS420441
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

CYP7B1, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-CYP7B1 Antibody

Product Synonym Names
CYP7B1; CP7B; cytochrome P450, family 7, subfamily B, polypeptide 1; oxysterol 7alpha-hydroxylase; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; CBAS3; SPG5A; CYP7B1 antibody; CP7B antibody; cytochrome P450; family 7; subfamily B; polypeptide 1 antibody; oxysterol 7alpha-hydroxylase antibody; cytochrome P450; subfamily VIIB (oxysterol 7 alpha-hydroxylase); polypeptide 1 antibody; CBAS3 antibody; SPG5A antibody
Antibody/Peptide Pairs
CYP7B1 peptide (MBS425932) is used for blocking the activity of CYP7B1 antibody (MBS420441)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
506
Immunogen Sequence
YPDSDVLFRY KVKS
OMIM
270800
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-YPDSDVLFRYKVKS, from the C Terminus of the protein sequence according to NP_004811.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CYP7B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CYP7B1 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-CYP7B1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Breast shows textured cytoplasm staining in ductal epithelial cells. Recommended concentration, 3-6ug/ml.
Western Blot: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).

Immunohistochemistry (IHC) of anti-CYP7B1 antibody
(4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-CYP7B1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CYP7B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
58,256 Da
NCBI Official Full Name
25-hydroxycholesterol 7-alpha-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450 family 7 subfamily B member 1
NCBI Official Symbol
CYP7B1  [Similar Products]
NCBI Official Synonym Symbols
CP7B; CBAS3; SPG5A
  [Similar Products]
NCBI Protein Information
25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Protein Name
25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Synonym Protein Names
Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase
UniProt Gene Name
CYP7B1  [Similar Products]
UniProt Entry Name
CP7B1_HUMAN
NCBI Summary for CYP7B1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]
UniProt Comments for CYP7B1
CYP7B1: Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3). Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. Belongs to the cytochrome P450 family.

Protein type: Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.100; Oxidoreductase

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: endoplasmic reticulum membrane

Molecular Function: 25-hydroxycholesterol 7alpha-hydroxylase activity; heme binding; iron ion binding; oxysterol 7-alpha-hydroxylase activity

Biological Process: bile acid biosynthetic process; cholesterol metabolic process; negative regulation of estrogen receptor signaling pathway; positive regulation of epithelial cell proliferation; sterol metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 3; Spastic Paraplegia 5a, Autosomal Recessive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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