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anti-FBXO32 antibody :: Goat FBXO32 Polyclonal Antibody

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Catalog # MBS421930
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

FBXO32, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-FBXO32 Antibody

Product Synonym Names
FBXO32; F-box protein 32; FLJ32424; Fbx32; MAFbx; MGC33610; F-box only protein 32; atrogin 1; muscle atrophy F-box protein; FBXO32 antibody; F-box protein 32 antibody; FLJ32424 antibody; Fbx32 antibody; MAFbx antibody; MGC33610 antibody; F-box only protein 32 antibody; atrogin 1 antibody; muscle atrophy F-box protein antibody
Antibody/Peptide Pairs
FBXO32 peptide (MBS426616) is used for blocking the activity of FBXO32 antibody (MBS421930)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
NSKTKTQYFH QEK
OMIM
606604
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-NSKTKTQYFHQEK, from the internal region of the protein sequence according to NP_478136.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoform 1 (NP_478136.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-FBXO32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FBXO32 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-FBXO32 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: approx. 40kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.6kDa according to Human NP_478136.1 and of 41.5kDa according to Mouse NP_080622.1). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-FBXO32 antibody
(1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-FBXO32 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for FBXO32. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,804 Da
NCBI Official Full Name
F-box only protein 32 isoform 1
NCBI Official Synonym Full Names
F-box protein 32
NCBI Official Symbol
FBXO32  [Similar Products]
NCBI Official Synonym Symbols
Fbx32; MAFbx
  [Similar Products]
NCBI Protein Information
F-box only protein 32
UniProt Protein Name
F-box only protein 32
UniProt Synonym Protein Names
Atrogin-1; Muscle atrophy F-box protein; MAFbx
Protein Family
UniProt Gene Name
FBXO32  [Similar Products]
UniProt Synonym Gene Names
MAFbx  [Similar Products]
UniProt Entry Name
FBX32_HUMAN
NCBI Summary for FBXO32
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
UniProt Comments for FBXO32
FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: cytoplasm; nucleoplasm; nucleus; SCF ubiquitin ligase complex; Z disc

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation; ubiquitin-dependent protein catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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