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anti-AAK1 antibody :: Goat AAK1 Polyclonal Antibody

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Catalog # MBS422033
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

AAK1, Polyclonal Antibody

Also Known As

Goat anti-AAK1 Antibody

Product Synonym Names
AAK1; AP2 associated kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570; adaptor-associated kinase 1; AAK1 antibody; AP2 associated kinase 1 antibody; DKFZp686F03202 antibody; DKFZp686K16132 antibody; FLJ23712 antibody; FLJ25931 antibody; FLJ31060 antibody; FLJ42882 antibody; FLJ45252 antibody; KIAA1048 antibody; MGC138170 antibody; MGC164568 antibody; MGC164570 antibody; adaptor-associated kinase 1 antibody
Antibody/Peptide Pairs
AAK1 peptide (MBS425104) is used for blocking the activity of AAK1 antibody (MBS422033)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
961
Immunogen Sequence
GSPRTSQQNV YNPSE
OMIM
616405
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-GSPRTSQQNVYNPSE, from the internal region of the protein sequence according to NP_055726.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-AAK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AAK1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-AAK1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 100kDa band observed in Human Brain (Amygdala and Cerebellum) lysates (calculated MW of 104kDa according to NP_055726.3). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-AAK1 antibody
(1ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-AAK1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AAK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
93,578 Da
NCBI Official Full Name
AP2-associated protein kinase 1
NCBI Official Synonym Full Names
AP2 associated kinase 1
NCBI Official Symbol
NCBI Protein Information
AP2-associated protein kinase 1
UniProt Protein Name
AP2-associated protein kinase 1
UniProt Synonym Protein Names
Adaptor-associated kinase 1
UniProt Gene Name
UniProt Synonym Gene Names
KIAA1048  [Similar Products]
UniProt Entry Name
AAK1_HUMAN
NCBI Summary for AAK1
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for AAK1
AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Other group; NAK family

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: clathrin-coated vesicle; coated pit; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button

Molecular Function: ATP binding; Notch binding; protein binding; protein serine/threonine kinase activity

Biological Process: endocytosis; positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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