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anti-ACAT1 antibody :: Goat ACAT1 Polyclonal Antibody

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Catalog # MBS422271
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

ACAT1, Polyclonal Antibody

Also Known As

Goat anti-ACAT1 (aa257-269) Antibody

Product Synonym Names
Goat Anti-ACAT1 (aa257-269), Biotinylated Antibody; ACAT1; acetyl-Coenzyme A acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; ACAT1 antibody; acetyl-Coenzyme A acetyltransferase 1 antibody; ACAT antibody; MAT antibody; T2 antibody; THIL antibody; acetoacetyl Coenzyme A thiolase antibody; ACAT1 antibody; acetyl-Coenzyme A acetyltransferase 1 antibody; ACAT antibody; MAT antibody; T2 antibody; THIL antibody; acetoacetyl Coenzyme A thiolase antibody; ACAT1 (aa257-269)
Matching Pairs
Unconjugated Antibody: ACAT1 (MBS422271)
Biotin Conjugated Antibody: ACAT1 (MBS423739)
Antibody/Peptide Pairs
ACAT1 peptide (MBS428284) is used for blocking the activity of ACAT1 antibody (MBS422271)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
427
Immunogen Sequence
KRVDFSKVPK LKT
OMIM
203750
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KRVDFSKVPKLKT, from the internal region of the protein sequence according to NP_000010.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ACAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACAT1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-ACAT1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 45kDa band observed in Human Liver lysates and in rodent Kidney and Liver lysates (calculated MW of 45.2kDa according to NP_000010.1). Recommended concentration: 0.01-0.03ug/ml.
Immunofluorescence: Customer finds particulate cytoplasm staining in HeLa.

Western Blot (WB) of anti-ACAT1 antibody
(0. 01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ACAT1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACAT1 antibody
(0. 01ug/ml) staining of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ACAT1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ACAT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,175 Da
NCBI Official Full Name
acetyl-CoA acetyltransferase, mitochondrial
NCBI Official Synonym Full Names
acetyl-CoA acetyltransferase 1
NCBI Official Symbol
ACAT1  [Similar Products]
NCBI Official Synonym Symbols
T2; MAT; ACAT; THIL
  [Similar Products]
NCBI Protein Information
acetyl-CoA acetyltransferase, mitochondrial
UniProt Protein Name
Acetyl-CoA acetyltransferase, mitochondrial
UniProt Synonym Protein Names
Acetoacetyl-CoA thiolase; T2
UniProt Gene Name
ACAT1  [Similar Products]
UniProt Synonym Gene Names
ACAT; MAT  [Similar Products]
UniProt Entry Name
THIL_HUMAN
NCBI Summary for ACAT1
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
UniProt Comments for ACAT1
ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Acetyltransferase; EC 2.3.1.9; Carbohydrate Metabolism - pyruvate; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - propanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: acetyl-CoA C-acetyltransferase activity; coenzyme binding; enzyme binding; metal ion binding; protein homodimerization activity

Biological Process: brain development; branched chain family amino acid catabolic process; ketone body biosynthetic process; ketone body catabolic process; liver development; protein homooligomerization; response to hormone stimulus; response to organic cyclic substance; response to starvation

Disease: Alpha-methylacetoacetic Aciduria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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