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anti-ACO1 antibody :: Goat ACO1/Aconitase 1 Polyclonal Antibody

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Catalog # MBS421648
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ACO1/Aconitase 1, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-ACO1/Aconitase 1 Antibody

Product Synonym Names
ACO1; aconitase 1, soluble; ACONS; IREB1; IREBP; IREBP1; IRP1; OTTHUMP00000021176; OTTHUMP00000021177; OTTHUMP00000045233; aconitase 1; aconitate hydratase; citrate hydro-lyase; ferritin repressor protein; iron regulatory protein 1; iron-responsive elemen; ACO1 antibody; aconitase 1; soluble antibody; ACONS antibody; IREB1 antibody; IREBP antibody; IREBP1 antibody; IRP1 antibody; OTTHUMP00000021176 antibody; OTTHUMP00000021177 antibody; OTTHUMP00000045233 antibody; aconitase 1 antibody; aconitate hydratase antibody; citrate hydro-lyase antibody; ferritin repressor protein antibody; iron regulatory protein 1 antibody; iron-responsive element binding protein 1 antibody; Aconitase 1; ACO1 / Aconitase 1
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
889
Immunogen Sequence
QVDFNRRADS LQKNQ
OMIM
100880
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QVDFNRRADSLQKNQ, from the internal region of the protein sequence according to NP_002188.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ACO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACO1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ACO1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 100kDa band observed in Human Liver lysates (calculated MW of 98.4kDa according to NP_002188.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-ACO1 antibody
(0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ACO1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ACO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
98,399 Da
NCBI Official Full Name
cytoplasmic aconitate hydratase
NCBI Official Synonym Full Names
aconitase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
  [Similar Products]
NCBI Protein Information
cytoplasmic aconitate hydratase
UniProt Protein Name
Cytoplasmic aconitate hydratase
UniProt Synonym Protein Names
Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IREB1; Aconitase; IRP1; IRE-BP 1  [Similar Products]
UniProt Entry Name
ACOC_HUMAN
NCBI Summary for ACO1
The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
UniProt Comments for ACO1
IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: RNA-binding; Endoplasmic reticulum; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Translation

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion

Molecular Function: 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; metal ion binding; protein binding; RNA binding

Biological Process: cellular iron ion homeostasis; citrate metabolic process; intestinal absorption; post-embryonic development; regulation of translation; response to iron(II) ion; tricarboxylic acid cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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