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anti-TP63 antibody :: Rabbit p40 (deltaNp63) Polyclonal Antibody

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Catalog # MBS438088
Unit / Price
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

p40 (deltaNp63) (TP63), Polyclonal Antibody

Popular Item
Also Known As

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody

Product Synonym Names
deltaNp63, TP63
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
555
OMIM
103285
3D Structure
ModBase 3D Structure for Q9H3D4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Cow. Others not known.
Specificity
p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
Form/Format
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Cellular Localization
Nuclear
Immunogen
A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40
Hu-Chromosome Location
3q28
Positive Control
HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-TP63 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TP63 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TP63 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilutions for a specific application should be determined.

Immunohistochemistry (IHC) of anti-TP63 antibody
Formalin-fixed, paraffin-embedded Prostate Carcinoma stained with p40 Rabbit Polyclonal Antibody.
anti-TP63 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry of anti-TP63 antibody
anti-TP63 antibody Immunohistochemistry (IHC) image
Western Blot of anti-TP63 antibody
anti-TP63 antibody Western Blot (WB) image
SDS-PAGE of anti-TP63 antibody
anti-TP63 antibody SDS-PAGE image
NCBI/Uniprot data below describe general gene information for TP63. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40kDa
NCBI Official Full Name
tumor protein 63 isoform 2
NCBI Official Synonym Full Names
tumor protein p63
NCBI Official Symbol
NCBI Official Synonym Symbols
AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
  [Similar Products]
NCBI Protein Information
tumor protein 63
UniProt Protein Name
Tumor protein 63
UniProt Synonym Protein Names
Chronic ulcerative stomatitis protein; CUSP; Keratinocyte transcription factor KET; Transformation-related protein 63; TP63; Tumor protein p73-like; p73L; p40; p51
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L  [Similar Products]
UniProt Entry Name
P63_HUMAN
NCBI Summary for TP63
This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2016]
UniProt Comments for TP63
p63: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Belongs to the p53 family. 12 isoforms of the human protein are produced by alternative promoter.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: cytoplasm; cytosol; dendrite; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: damaged DNA binding; double-stranded DNA binding; identical protein binding; p53 binding; protein binding; sequence-specific DNA binding; transcription factor activity; WW domain binding

Biological Process: apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; G1 DNA damage checkpoint; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Notch signaling pathway; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein homotetramerization; regulation of apoptosis; regulation of epidermal cell division; regulation of neuron apoptosis; response to gamma radiation; response to X-ray

Disease: Adult Syndrome; Ankyloblepharon-ectodermal Defects-cleft Lip/palate; Ectrodactyly, Ectodermal Dysplasia, And Cleft Lip/palate Syndrome 3; Limb-mammary Syndrome; Rapp-hodgkin Syndrome; Split-hand/foot Malformation 4
Product References and Citations for anti-TP63 antibody
Bishop, JA et. al. Modern Pathology 25 : 405-4152. Scagliotti G et. al. J Thorac Oncol 6:64-70. 3. Kargi A et. al. Appl Immunohistochem Mol Morphol 15:415-420

Precautions
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Disclaimer
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