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anti-PDXP antibody :: Goat PDXP Polyclonal Antibody

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Catalog # MBS422888
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

PDXP, Polyclonal Antibody

Full Product Name

Goat anti-PDXP Antibody

Product Synonym Names
PDXP; pyridoxal (pyridoxine, vitamin B6) phosphatase; CIN; FLJ32703; PLP; dJ37E16.5; chronophin; pyridoxal phosphatase; pyridoxal phosphate phosphatase; chronophin antibody; CIN antibody; dJ37E16.5 antibody; FLJ32703 antibody; PLP antibody; pyridoxal (pyridoxine; vitamin B6) phosphatase antibody; pyridoxal phosphatase antibody; pyridoxal phosphate phosphatase antibody; PDXP antibody
Antibody/Peptide Pairs
PDXP peptide (MBS427672) is used for blocking the activity of PDXP antibody (MBS422888)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
QHDLVPHYYV ES
OMIM
609246
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QHDLVPHYYVES, from the C Terminus of the protein sequence according to NP_064711.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PDXP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PDXP antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PDXP antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 32kDa band observed in Human and Rat Heart lysates and in Mouse and Rat Brain lysates (calculated MW of 31.7kDa according to NP_064711.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-PDXP antibody
(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PDXP antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-PDXP antibody
(0.1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PDXP antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PDXP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,698 Da
NCBI Official Full Name
pyridoxal phosphate phosphatase
NCBI Official Synonym Full Names
pyridoxal phosphatase
NCBI Official Symbol
NCBI Official Synonym Symbols
CIN; PLP; dJ37E16.5
  [Similar Products]
NCBI Protein Information
pyridoxal phosphate phosphatase
UniProt Protein Name
Pyridoxal phosphate phosphatase
UniProt Synonym Protein Names
Chronophin
UniProt Gene Name
UniProt Synonym Gene Names
CIN; PLP; PLPP; PLP phosphatase  [Similar Products]
UniProt Entry Name
PLPP_HUMAN
NCBI Summary for PDXP
Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP (Jang et al., 2003 [PubMed 14522954]).[supplied by OMIM, Mar 2008]
UniProt Comments for PDXP
PDXP: Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho- tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP. Homodimer. Ubiquitous. Highly expressed in all the regions of central nerve system except the spinal cord. Also expressed at high level in liver and testis. In fetus, it is weakly expressed in all organs except brain. Inhibited by NaF, Zn(2+), Ca(2+), Mn(2+) and EDTA. Belongs to the HAD-like hydrolase superfamily.

Protein type: Cofactor and Vitamin Metabolism - vitamin B6; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.74; EC 3.1.3.3; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: actin cytoskeleton; cleavage furrow; cytosol; lamellipodium; midbody; plasma membrane

Molecular Function: heat shock protein binding; magnesium ion binding; phosphoprotein phosphatase activity; phosphoserine phosphatase activity; protein binding; pyridoxal phosphatase activity

Biological Process: actin rod formation; positive regulation of actin filament depolymerization; protein amino acid dephosphorylation; pyridoxal phosphate catabolic process; regulation of cytokinesis; regulation of mitosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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