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anti-TERF1 antibody :: Goat TERF1/PIN2 Polyclonal Antibody

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Catalog # MBS420396
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TERF1/PIN2, Polyclonal Antibody

Also Known As

Goat anti-TERF1/PIN2 Antibody

Product Synonym Names
Goat Anti-TERF1 / TRF1 Antibody; TERF1; PIN2; TRF; TRF1; TRBF1; hTRF1-AS; telomeric repeat binding factor (NIMA-interacting) 1; Telomeric repeat binding factor 1; telomeric repeat binding protein 1; FLJ41416; t-TRF1; TERF1 antibody; PIN2 antibody; TRF antibody; TRF1 antibody; TRBF1 antibody; hTRF1-AS antibody; telomeric repeat binding factor (NIMA-interacting) 1 antibody; Telomeric repeat binding factor 1 antibody; telomeric repeat binding protein 1 antibody; FLJ41416 antibody; t-TRF1 antibody; TERF1 / PIN2
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
419
Immunogen Sequence
TMKKLKLISS DSED
OMIM
600951
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-TMKKLKLISSDSED, from the C Terminus of the protein sequence according to NP_003209.2; NP_059523.2.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform 1 (NP_059523.2) and isoform 2 (NP_003209.2).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-TERF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TERF1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-TERF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 60-65kDa and 45kDa bands seen in HeLa lysate. Recommended for use at 1-3ug/ml.

Western Blot (WB) of anti-TERF1 antibody
Staining (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
anti-TERF1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TERF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_059523.2[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48,239 Da
NCBI Official Full Name
telomeric repeat-binding factor 1 isoform 2
NCBI Official Synonym Full Names
telomeric repeat binding factor 1
NCBI Official Symbol
TERF1  [Similar Products]
NCBI Official Synonym Symbols
TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
  [Similar Products]
NCBI Protein Information
telomeric repeat-binding factor 1
UniProt Protein Name
Telomeric repeat-binding factor 1
UniProt Synonym Protein Names
NIMA-interacting protein 2; TTAGGG repeat-binding factor 1; Telomeric protein Pin2/TRF1
UniProt Gene Name
TERF1  [Similar Products]
UniProt Synonym Gene Names
PIN2; TRBF1; TRF; TRF1  [Similar Products]
UniProt Entry Name
TERF1_HUMAN
NCBI Summary for TERF1
This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]
UniProt Comments for TERF1
TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: chromosome, telomeric region; cytoplasm; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleolus; nucleoplasm; nucleus; spindle

Molecular Function: DNA bending activity; DNA binding; double-stranded telomeric DNA binding; microtubule binding; protein binding; protein heterodimerization activity; protein homodimerization activity; telomerase activity; telomeric DNA binding; ubiquitin binding

Biological Process: cell division; G2/M transition of mitotic cell cycle; mitosis; mitotic cell cycle spindle assembly checkpoint; negative regulation of DNA replication; negative regulation of telomerase activity; negative regulation of telomere maintenance via semi-conservative replication; negative regulation of telomere maintenance via telomerase; positive regulation of apoptosis; positive regulation of microtubule polymerization; positive regulation of mitosis; positive regulation of mitotic cell cycle; protein homooligomerization; response to drug; telomere capping; telomere clustering; telomere maintenance; telomere maintenance via telomerase; telomeric loop formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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