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anti-SMO antibody :: Goat SMO Polyclonal Antibody

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Catalog # MBS420961
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

SMO, Polyclonal Antibody

Also Known As

Goat anti-SMO (internal) Antibody

Product Synonym Names
Goat Anti-SMO (C Terminus) Antibody; SMO; smoothened homolog (Drosophila); Gx; SMOH; seven transmembrane helix receptor; smoothened; SMO antibody; smoothened homolog (Drosophila) antibody; Gx antibody; SMOH antibody; seven transmembrane helix receptor antibody; smoothened antibody; SMO (internal)
Product Gene Name
Antibody/Peptide Pairs
SMO peptide (MBS426743) is used for blocking the activity of SMO antibody (MBS420961)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
787
Immunogen Sequence
QSDDEPKRIK KS
OMIM
601500
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Zebrafish
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QSDDEPKRIKKS, from the internal region of the protein sequence according to NP_005622.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SMO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SMO antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SMO antibody
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 60kDa band observed in Human Bone Marrow lysates (calculated MW of 86.4kDa according to NP_005622.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-SMO antibody
A) and B) (0.3ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SMO antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SMO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,397 Da
NCBI Official Full Name
smoothened homolog
NCBI Official Synonym Full Names
smoothened, frizzled class receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
Gx; SMOH; FZD11
  [Similar Products]
NCBI Protein Information
smoothened homolog
UniProt Protein Name
Smoothened homolog
UniProt Synonym Protein Names
Protein Gx
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SMOH; SMO  [Similar Products]
UniProt Entry Name
SMO_HUMAN
NCBI Summary for SMO
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
UniProt Comments for SMO
SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; Oncoprotein; GPCR, Fz/Smo family; Membrane protein, multi-pass; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q32.3

Cellular Component: caveola; Golgi apparatus; integral to membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: drug binding; G-protein coupled receptor activity; patched binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: activation of hh target transcription factor; anterior/posterior pattern formation; astrocyte activation; cell fate specification; central nervous system neuron differentiation; cerebellar cortex morphogenesis; cerebral cortex development; dentate gyrus development; dorsoventral neural tube patterning; forebrain morphogenesis; G-protein coupled receptor protein signaling pathway; hair follicle morphogenesis; heart looping; homeostasis of number of cells within a tissue; in utero embryonic development; midgut development; multicellular organism growth; myoblast migration; negative regulation of apoptosis; negative regulation of DNA binding; negative regulation of epithelial cell differentiation; negative regulation of hair follicle development; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of multicellular organism growth; positive regulation of neuroblast proliferation; positive regulation of organ growth; positive regulation of protein import into nucleus; positive regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; protein stabilization; skeletal muscle fiber development; smoothened signaling pathway; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; smoothened signaling pathway in ventral spinal cord patterning; thalamus development; vasculogenesis; ventral midline determination; Wnt receptor signaling pathway through beta-catenin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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