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anti-VIL2 antibody :: Goat Ezrin/villin 2 Polyclonal Antibody

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Catalog # MBS420129
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

Ezrin/villin 2 (VIL2), Polyclonal Antibody

Popular Item
Also Known As

Goat anti-Ezrin/villin 2 Antibody

Product Synonym Names
villin 2 (ezrin); ezrin; VIL2; CVIL; CVL; DKFZp762H157; FLJ26216; MGC1584; cytovillin; villin 2; villin 2 (ezrin) antibody; ezrin antibody; VIL2 antibody; CVIL antibody; CVL antibody; DKFZp762H157 antibody; FLJ26216 antibody; MGC1584 antibody; cytovillin antibody; villin 2 antibody; Ezrin; Villin 2; Ezrin / villin 2
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
586
Immunogen Sequence
QDYEEKTKKA ER
OMIM
123900
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen
Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-VIL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-VIL2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-VIL2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.


Western Blot: Approx 80kDa band observed in Human Placenta Lysates.(calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol.2005 Jun;288(6): C1242-54.; PMID: 15788482). Recommended concentration: 1-3ug/ml.

Immunohistochemistry: In paraffin embedded Human Small Intestine shows strong staining of enterocytes.
Recommended concentration, 3-5ug/ml.Paraffin embedded Human Kidney.
Recommended concentration: 3.75 mg/mL

Western Blot (WB) of anti-VIL2 antibody
(1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-VIL2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-VIL2 antibody
(3.8ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-VIL2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for VIL2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,413 Da
NCBI Official Full Name
ezrin
NCBI Official Synonym Full Names
ezrin
NCBI Official Symbol
NCBI Official Synonym Symbols
CVL; CVIL; VIL2; HEL-S-105
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NCBI Protein Information
ezrin
UniProt Protein Name
Ezrin
UniProt Synonym Protein Names
Cytovillin; Villin-2; p81
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
EZRI_HUMAN
NCBI Summary for VIL2
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for VIL2
Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: actin cytoskeleton; actin filament; apical part of cell; apical plasma membrane; basolateral plasma membrane; brush border; cell projection; cortical cytoskeleton; cytoplasm; cytosol; endosome; extracellular space; extrinsic to membrane; filopodium; focal adhesion; immunological synapse; membrane; microvillus; microvillus membrane; myelin sheath; nucleolus; perinuclear region of cytoplasm; plasma membrane; ruffle; T-tubule; uropod; vesicle

Molecular Function: actin binding; actin filament binding; ATPase binding; cell adhesion molecule binding; microtubule binding; protein binding; protein domain specific binding

Biological Process: actin cytoskeleton reorganization; actin filament bundle formation; astral microtubule organization and biogenesis; axon guidance; cortical microtubule organization and biogenesis; cytoskeletal anchoring; D-glucose absorption; establishment of centrosome localization; establishment of epithelial cell polarity; filopodium formation; gland morphogenesis; leukocyte adhesion; membrane to membrane docking; microvillus biogenesis; negative regulation of T cell receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; positive regulation of multicellular organism growth; positive regulation of protein secretion; receptor internalization; regulation of actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of cell size; regulation of microvillus length
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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