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anti-CBX5 antibody :: Goat CBX5/HP1-Alpha Polyclonal Antibody

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Catalog # MBS420702
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

CBX5/HP1-Alpha, Polyclonal Antibody

Also Known As

Goat anti-CBX5/HP1-Alpha Antibody

Product Synonym Names
CBX5; HP1-ALPHA; chromobox homolog 5 (HP1 alpha homolog, Drosophila); HGNC: 1555; HP1; HP1Hs-alpha; chromobox homolog 5 (Drosophila HP1 alpha); Heterochromatin protein-1; HP1A; antigen p25; heterochromatin protein 1 homolog alpha; heterochromatin protein; CBX5 antibody; HP1-ALPHA antibody; chromobox homolog 5 (HP1 alpha homolog; Drosophila) antibody; HGNC: 1555 antibody; HP1 antibody; HP1Hs-alpha antibody; chromobox homolog 5 (Drosophila HP1 alpha) antibody; Heterochromatin protein-1 antibody; HP1A antibody; antigen p25 antibody; heterochromatin protein 1 homolog alpha antibody; heterochromatin protein 1-alpha antibody; CBX5 / HP1-Alpha
Product Synonym Gene Name
HP1-Alpha[Similar Products]
Matching Pairs
Unconjugated Antibody: CBX5/HP1-Alpha (MBS420702)
Biotin Conjugated Antibody: CBX5/HP1-Alpha (MBS423571)
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
191
Immunogen Sequence
NKRKSNFSNS ADDIK
OMIM
604478
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1; NP_001120793.1; NP_001120794.1.
Epitope
Internal region
Note
Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CBX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CBX5 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CBX5 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Uterus shows strong nuclear staining in the glandular epithelial cells. Recommended concentration, 2-3ug/ml.
Western Blot: Approx.22kDa band observed in lysates of Human fibroblast cell lines A431 and HeLa (calculated MW of 22.2kDa according to NP_036249.1). Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-CBX5 antibody
(0. 03ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CBX5 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CBX5 antibody
(2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-CBX5 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CBX5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001120793.1; NP_001120794.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,225 Da
NCBI Official Full Name
chromobox protein homolog 5
NCBI Official Synonym Full Names
chromobox 5
NCBI Official Symbol
NCBI Official Synonym Symbols
HP1; HP1A; HEL25
  [Similar Products]
NCBI Protein Information
chromobox protein homolog 5
UniProt Protein Name
Chromobox protein homolog 5
UniProt Synonym Protein Names
Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HP1A; HP1 alpha  [Similar Products]
UniProt Entry Name
CBX5_HUMAN
NCBI Summary for CBX5
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for CBX5
HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: centric heterochromatin; chromocenter; histone deacetylase complex; histone methyltransferase complex; kinetochore; nuclear chromosome, telomeric region; nuclear envelope; nuclear heterochromatin; nucleolus; nucleoplasm; nucleus; PML body; transcriptional repressor complex

Molecular Function: chromatin binding; histone deacetylase binding; methylated histone residue binding; protein binding; protein binding, bridging; protein homodimerization activity

Biological Process: blood coagulation; negative regulation of transcription, DNA-dependent; viral reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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