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anti-SFN antibody :: Goat 14-3-3 sigma/Stratifin Polyclonal Antibody

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Catalog # MBS421444
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

14-3-3 sigma/Stratifin (SFN), Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-14-3-3 sigma/Stratifin Antibody

Product Synonym Names
SFN; 14-3-3sigma; stratifin; HGNC: 10773; YWHAS; 14-3-3sigma antibody; stratifin antibody; HGNC: 10773 antibody; SFN antibody; YWHAS antibody; 14-3-3 sigma / Stratifin
Product Gene Name
Product Synonym Gene Name
14-3-3 sigma; Stratifin[Similar Products]
Matching Pairs
Unconjugated Antibody: 14-3-3 sigma/Stratifin (MBS421444)
Biotin Conjugated Antibody: 14-3-3 sigma/Stratifin (MBS423714)
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
DLHTLSEDSY KDST
OMIM
601290
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.
Epitope
Internal region
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7681
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SFN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SFN antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SFN antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4ug/ml.
Western Blot: Approx.28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-SFN antibody
(0. 03ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SFN antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SFN antibody
(2ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
anti-SFN antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for SFN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,336 Da
NCBI Official Full Name
14-3-3 protein sigma
NCBI Official Synonym Full Names
stratifin
NCBI Official Symbol
NCBI Official Synonym Symbols
YWHAS
  [Similar Products]
NCBI Protein Information
14-3-3 protein sigma
UniProt Protein Name
14-3-3 protein sigma
UniProt Synonym Protein Names
Epithelial cell marker protein 1; Stratifin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
1433S_HUMAN
UniProt Comments for SFN
14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space; mitochondrion; nucleus

Molecular Function: phosphoprotein binding; protein binding; protein domain specific binding; protein kinase binding; protein kinase C inhibitor activity

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; negative regulation of caspase activity; negative regulation of protein kinase activity; positive regulation of cell growth; positive regulation of epidermal cell differentiation; positive regulation of protein export from nucleus; regulation of cyclin-dependent protein kinase activity; regulation of epidermal cell division; release of cytochrome c from mitochondria; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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