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anti-AKAP10 antibody :: Goat AKAP10/D-AKAP2 Polyclonal Antibody

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Catalog # MBS420206
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

AKAP10/D-AKAP2, Polyclonal Antibody

Also Known As

Goat anti-AKAP10/D-AKAP2 Antibody

Product Synonym Names
Goat Anti-AKAP10 Antibody; AKAP10; PRKA10; D-AKAP2; MGC: 9414; A kinase (PRKA) anchor protein 10; dual specificity A-Kinase anchoring protein 2; A-kinase anchor protein 10; dual-specificity A-kinase anchoring protein 2; mitochondrial A kinase PPKA anchor protein 10; protein kinase A; AKAP10 antibody; PRKA10 antibody; D-AKAP2 antibody; MGC: 9414 antibody; A kinase (PRKA) anchor protein 10 antibody; dual specificity A-Kinase anchoring protein 2 antibody; A-kinase anchor protein 10 antibody; dual-specificity A-kinase anchoring protein 2 antibody; mitochondrial A kinase PPKA anchor protein 10 antibody; protein kinase A anchoring protein 10 antibody; AKAP10 / D-AKAP2
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
662
Immunogen Sequence
AQYDQPLEKS TKL
OMIM
115080
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-AQYDQPLEKSTKL, from the C Terminus of the protein sequence according to NP_009133.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-AKAP10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AKAP10 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-AKAP10 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 65kDa band observed in Human Testis lysate (Calculated MW of 73.8kDa according to NP_009133.2) Recommended concnetration: 0.3-1. 0ug/ml.

Western Blot (WB) of anti-AKAP10 antibody
(0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer. Primary incubation was 1 hour. Deected by chemiluminescence.
anti-AKAP10 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AKAP10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,818 Da
NCBI Official Full Name
A-kinase anchor protein 10, mitochondrial
NCBI Official Synonym Full Names
A-kinase anchoring protein 10
NCBI Official Symbol
AKAP10  [Similar Products]
NCBI Official Synonym Symbols
PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
  [Similar Products]
NCBI Protein Information
A-kinase anchor protein 10, mitochondrial
UniProt Protein Name
A-kinase anchor protein 10, mitochondrial
UniProt Synonym Protein Names
Dual specificity A kinase-anchoring protein 2; D-AKAP-2; Protein kinase A-anchoring protein 10; PRKA10
Protein Family
UniProt Gene Name
AKAP10  [Similar Products]
UniProt Synonym Gene Names
AKAP-10; D-AKAP-2; PRKA10  [Similar Products]
UniProt Entry Name
AKA10_HUMAN
NCBI Summary for AKAP10
This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2012]
UniProt Comments for AKAP10
AKAP10: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase. Genetic variations in AKAP10 are a cause of susceptibility to sudden cardiac death (SCD). Unexpected rapid natural death due to cardiovascular collapse within one hour of initial symptoms. It is usually caused by the worsening of existing heart diseases. The sudden onset of symptoms, such as chest pain and cardiac arrhythmias, particularly ventricular tachycardia, can lead to the loss of consciousness and cardiac arrest followed by biological death. Increased susceptibility to sudden cardiac death may be conferred by AKAP10 variants that are associated with markers of low vagus nerve sensitivity, e.g. fast basal heart rate and low heart rate variability.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p11.1

Cellular Component: cytoplasm; cytosol; mitochondrion; plasma membrane

Biological Process: blood coagulation; protein localization; signal transduction

Disease: Cardiac Conduction Defect
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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