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anti-BHMT antibody :: Goat BHMT Polyclonal Antibody

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Catalog # MBS421546
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

BHMT, Polyclonal Antibody

Full Product Name

Goat anti-BHMT Antibody

Product Synonym Names
BHMT; betaine-homocysteine methyltransferase; BHMT antibody; betaine-homocysteine methyltransferase antibody
Antibody/Peptide Pairs
BHMT peptide (MBS425943) is used for blocking the activity of BHMT antibody (MBS421546)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
EQQLKELFEK QK
OMIM
602888
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-EQQLKELFEKQK, from the C Terminus of the protein sequence according to NP_001704.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-BHMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BHMT antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BHMT antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45. 0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-BHMT antibody
(0. 03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-BHMT antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-BHMT antibody
HEK293 overexpressing BHMT RC203148) and probed (mock transfection in first lane), tested by Origene.
anti-BHMT antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BHMT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,998 Da
NCBI Official Full Name
betaine-homocysteine methyltransferase
NCBI Official Synonym Full Names
betaine--homocysteine S-methyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
BHMT1; HEL-S-61p
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NCBI Protein Information
betaine--homocysteine S-methyltransferase 1
UniProt Protein Name
Betaine--homocysteine S-methyltransferase 1
UniProt Gene Name
UniProt Entry Name
BHMT1_HUMAN
NCBI Summary for BHMT
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
UniProt Comments for BHMT
BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Amino Acid Metabolism - cysteine and methionine; Methyltransferase; EC 2.1.1.5; Amino Acid Metabolism - glycine, serine and threonine

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding

Biological Process: betaine catabolic process; betaine metabolic process; choline catabolic process; protein amino acid methylation; regulation of homocysteine metabolic process; S-adenosylmethionine metabolic process; sulfur amino acid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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