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anti-CTNNA1 antibody :: Goat Catenin alpha1 Polyclonal Antibody

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Catalog # MBS421290
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

Catenin alpha1 (CTNNA1), Polyclonal Antibody

Also Known As

Goat anti-Catenin alpha1 Antibody

Product Synonym Names
catenin (cadherin-associated protein), alpha 1, 102kDa; HGNC: 2509; CTNNA1; CAP102; FLJ36832; alpha 1 (102kD); alpha-E-catenin; alpha-catenin; alphaE-catenin; cadherin-associated protein,102kDa; catenin (cadherin-associated protein), alpha 1 (102kD); cat; catenin (cadherin-associated protein); alpha 1; 102kDa antibody; HGNC: 2509 antibody; CTNNA1 antibody; CAP102 antibody; FLJ36832 antibody; alpha 1 (102kD) antibody; alpha-E-catenin antibody; alpha-catenin antibody; alphaE-catenin antibody; cadherin-associated protein; 102kDa antibody; catenin (cadherin-associated protein); alpha 1 (102kD) antibody; catenin; alpha 1 antibody; Catenin alpha1; Catenin alpha1
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
906
Immunogen Sequence
KREKQDETQT KIK
OMIM
116805
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KREKQDETQTKIK, from the internal region of the protein sequence according to NP_001894.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-CTNNA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CTNNA1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CTNNA1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Prostate shows staining of cell membranes of the secretory cells in the glands. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 100kDa band observed in human colon and human skin lysates (calculated MW of 100kDa according to NP_001894.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-CTNNA1 antibody
(1ug/ml) staining of human colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CTNNA1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-CTNNA1 antibody
HEK293 overexpressing Human CTNNA1 (RC201766) and probed (mock transfection in first lane), tested by Origene.
anti-CTNNA1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CTNNA1 antibody
(3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-CTNNA1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CTNNA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,550 Da
NCBI Official Full Name
catenin alpha-1 isoform 1
NCBI Official Synonym Full Names
catenin alpha 1
NCBI Official Symbol
CTNNA1  [Similar Products]
NCBI Official Synonym Symbols
MDPT2; CAP102
  [Similar Products]
NCBI Protein Information
catenin alpha-1
UniProt Protein Name
Catenin alpha-1
UniProt Synonym Protein Names
Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13
Protein Family
UniProt Gene Name
CTNNA1  [Similar Products]
UniProt Entry Name
CTNA1_HUMAN
NCBI Summary for CTNNA1
This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]
UniProt Comments for CTNNA1
CTNNA1: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F- actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Expressed ubiquitously in normal tissues. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: acrosome; actin cytoskeleton; catenin complex; cell junction; cytosol; flotillin complex; focal adhesion; Golgi apparatus; intercellular junction; intracellular membrane-bound organelle; lamellipodium; plasma membrane; zonula adherens

Molecular Function: actin filament binding; beta-catenin binding; cadherin binding; gamma-catenin binding; protein binding; structural molecule activity; vinculin binding

Biological Process: actin filament organization; aging; apical junction assembly; axon regeneration; cell adhesion; establishment and/or maintenance of cell polarity; gap junction assembly; male gonad development; negative regulation of neuroblast proliferation; odontogenesis of dentine-containing teeth; ovarian follicle development; positive regulation of muscle cell differentiation; positive regulation of smoothened signaling pathway; protein heterooligomerization; response to estrogen stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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