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anti-DSCAM antibody :: Goat DSCAM Polyclonal Antibody

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Catalog # MBS420303
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

DSCAM, Polyclonal Antibody

Full Product Name

Goat anti-DSCAM Antibody

Product Synonym Names
DSCAM; Down syndrome cell adhesion molecule; CHD2-42; CHD2-52; human CHD2-52 down syndrome cell adhesion molecule; DSCAM antibody; Down syndrome cell adhesion molecule antibody; CHD2-42 antibody; CHD2-52 antibody; human CHD2-52 down syndrome cell adhesion molecule antibody
Antibody/Peptide Pairs
DSCAM peptide (MBS426529) is used for blocking the activity of DSCAM antibody (MBS420303)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
DSWDSAQRTK DVSPQ
OMIM
602523
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DSWDSAQRTKDVSPQ, from the internal region of the protein sequence according to NP_001380.2; NP_996770.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-DSCAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-DSCAM antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-DSCAM antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 174kDa according to NP_996770.1). Recommended concentration: 2-6ug/ml.

Western Blot (WB) of anti-DSCAM antibody
(2ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-DSCAM antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DSCAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_996770.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
173,804 Da
NCBI Official Full Name
Down syndrome cell adhesion molecule isoform CHD2-42
NCBI Official Synonym Full Names
DS cell adhesion molecule
NCBI Official Symbol
DSCAM  [Similar Products]
NCBI Official Synonym Symbols
CHD2; CHD2-42; CHD2-52
  [Similar Products]
NCBI Protein Information
Down syndrome cell adhesion molecule
UniProt Protein Name
Down syndrome cell adhesion molecule
UniProt Synonym Protein Names
CHD2
UniProt Gene Name
DSCAM  [Similar Products]
UniProt Entry Name
DSCAM_HUMAN
NCBI Summary for DSCAM
This gene is a member of the immunoglobulin superfamily of cell adhesion molecules (Ig-CAMs), and is involved in human central and peripheral nervous system development. This gene is a candidate for Down syndrome and congenital heart disease (DSCHD). A gene encoding a similar Ig-CAM protein is located on chromosome 11. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]
UniProt Comments for DSCAM
DSCAM: Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: axon; cell junction; extracellular region; growth cone; integral to plasma membrane; membrane; plasma membrane; synapse

Molecular Function: protein binding

Biological Process: cell adhesion; dendrite morphogenesis; homophilic cell adhesion; locomotory behavior; negative regulation of cell adhesion; nervous system development; positive regulation of axon extension involved in axon guidance; positive regulation of phosphorylation; post-embryonic retina morphogenesis in camera-type eye; synaptogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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