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anti-G6PD antibody :: Goat G6PD Polyclonal Antibody

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Catalog # MBS420736
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

G6PD, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-G6PD (aa 308 - 320) Antibody

Product Synonym Names
Goat Anti-G6PD (aa 305 - 318) Antibody; G6PD; glucose-6-phosphate dehydrogenase; G6PD1; glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase; G6PD; G6PD antibody; glucose-6-phosphate dehydrogenase antibody; G6PD1 antibody; glucose-6-phosphate 1-dehydrogenase antibody; glucose-6-phosphate dehydrogenase antibody; G6PD antibody; G6PD (aa 308 - 320)
Antibody/Peptide Pairs
G6PD peptide (MBS425310) is used for blocking the activity of G6PD antibody (MBS420736)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
STNSDDVRDE KVK
OMIM
300908
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 ; NP_001035810.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-G6PD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-G6PD antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-G6PD antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Testis shows strong cytoplasm staining in spermatogonia. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0. 03-0.1ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml.

Western Blot (WB) of anti-G6PD antibody
(0. 03ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-G6PD antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-G6PD antibody
(2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-G6PD antibody Immunohistochemistry (IHC) (IHC) image
Testing Data of anti-G6PD antibody
(5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).
anti-G6PD antibody Testing Data image
NCBI/Uniprot data below describe general gene information for G6PD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001035810.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,468 Da
NCBI Official Full Name
glucose-6-phosphate 1-dehydrogenase isoform a
NCBI Official Synonym Full Names
glucose-6-phosphate dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
G6PD1
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NCBI Protein Information
glucose-6-phosphate 1-dehydrogenase
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
G6PD_HUMAN
NCBI Summary for G6PD
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for G6PD
G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: Other Amino Acids Metabolism - glutathione; Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.49; Oxidoreductase; Cell development/differentiation

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centrosome; cytoplasm; cytosol; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; microtubule organizing center; nucleus

Molecular Function: glucose binding; glucose-6-phosphate dehydrogenase activity; identical protein binding; NADP binding; protein binding; protein homodimerization activity

Biological Process: cholesterol biosynthetic process; erythrocyte maturation; glucose 6-phosphate metabolic process; glucose metabolic process; glutathione metabolic process; lipid metabolic process; NADP metabolic process; NADPH regeneration; pentose biosynthetic process; pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; regulation of neuron apoptosis; response to ethanol; response to food; response to organic cyclic substance; ribose phosphate biosynthetic process; substantia nigra development

Disease: Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Favism, Susceptibility To; Malaria, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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