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anti-GSN antibody :: Goat Gelsolin/GSN Polyclonal Antibody

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Catalog # MBS420495
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Gelsolin/GSN, Polyclonal Antibody

Also Known As

Goat anti-Gelsolin/GSN Antibody

Product Synonym Names
Goat Anti-Gelsolin (isoform a) Antibody; GSN; gelsolin; gelsolin (amyloidosis, Finnish type); DKFZp313L0718; GSN antibody; gelsolin antibody; gelsolin (amyloidosis; Finnish type) antibody; DKFZp313L0718 antibody; Gelsolin; Gelsolin / GSN
Product Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
782
Immunogen Sequence
PRLKDKKMDA HP
OMIM
105120
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-PRLKDKKMDAHP, from the internal region of the protein sequence according to NP_000168.1; NP_937895.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_000168.1 and NP_937895.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GSN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GSN antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GSN antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 90kDa band observed in Human Spleen lysates (calculated MW of 85.7kDa according to NP_000168.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-GSN antibody
(0.1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-GSN antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for GSN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_937895.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,485 Da
NCBI Official Full Name
gelsolin isoform a
NCBI Official Synonym Full Names
gelsolin
NCBI Official Symbol
NCBI Official Synonym Symbols
ADF; AGEL
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NCBI Protein Information
gelsolin
UniProt Protein Name
Gelsolin
UniProt Synonym Protein Names
AGEL; Actin-depolymerizing factor; ADF; Brevin
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
GELS_HUMAN
NCBI Summary for GSN
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for GSN
Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.

Protein type: Secreted; Actin-binding; Secreted, signal peptide; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: actin cap; actin cytoskeleton; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; ruffle; sarcoplasm

Molecular Function: actin binding; calcium ion binding; myosin II binding; protein binding; protein domain specific binding

Biological Process: actin filament capping; actin filament polymerization; actin filament severing; actin nucleation; aging; barbed-end actin filament capping; cellular protein metabolic process; extracellular matrix disassembly; negative regulation of virion penetration into host cell; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; protein destabilization; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration

Disease: Amyloidosis, Finnish Type
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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