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anti-IDO1 antibody :: Goat INDO/IDO Polyclonal Antibody

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Catalog # MBS421061
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

INDO/IDO (IDO1), Polyclonal Antibody

Also Known As

Goat anti-INDO/IDO Antibody

Product Synonym Names
IDO1; INDO; indoleamine-pyrrole 2,3 dioxygenase; HGNC: 6059; CD107B; IDO; Indoleamine 2,3-dioxygenase; indole 2,3-dioxygenase; INDO antibody; indoleamine-pyrrole 2; 3 dioxygenase antibody; HGNC: 6059 antibody; CD107B antibody; IDO antibody; Indoleamine 2; 3-dioxygenase antibody; indole 2; 3-dioxygenase antibody; IDO1 antibody; INDO / IDO
Product Synonym Gene Name
INDO; IDO[Similar Products]
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
403
Immunogen Sequence
CVLANWKKKD PNK
OMIM
147435
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CVLANWKKKDPNK, from the internal region of the protein sequence according to NP_002155.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-IDO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IDO1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IDO1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Colon shows textured staining in the cytoplasm of absorptive cells. Recommended concentration, 4-6ug/ml. Data obtained from previous batch (different goat).
Western Blot: Approx 50kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3kDa according to NP_002155.1). Recommended concentration: 0.5-1.5ug/ml.

Western Blot (WB) of anti-IDO1 antibody
(0.5ug/ml) staining of Human Lymph Node (A), Spleen (B) and Placenta (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-IDO1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-IDO1 antibody
(4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-IDO1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for IDO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,326 Da
NCBI Official Full Name
indoleamine 2,3-dioxygenase 1
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
IDO; INDO; IDO-1
  [Similar Products]
NCBI Protein Information
indoleamine 2,3-dioxygenase 1
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Synonym Protein Names
Indoleamine-pyrrole 2,3-dioxygenase
UniProt Gene Name
UniProt Synonym Gene Names
IDO; INDO; IDO-1  [Similar Products]
UniProt Entry Name
I23O1_HUMAN
NCBI Summary for IDO1
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]
UniProt Comments for IDO1
INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; Cell cycle regulation; EC 1.13.11.52

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: cytosol; smooth muscle contractile fiber; stereocilium bundle

Molecular Function: electron carrier activity; heme binding; indoleamine 2,3-dioxygenase activity; metal ion binding; tryptophan 2,3-dioxygenase activity

Biological Process: cytokine production during acute inflammatory response; female pregnancy; immune system process; multicellular organismal response to stress; negative regulation of interleukin-10 production; negative regulation of T cell proliferation; positive regulation of chronic inflammatory response; positive regulation of interleukin-12 production; positive regulation of T cell tolerance induction; positive regulation of T-helper 2 type immune response; regulation of activated T cell proliferation; response to lipopolysaccharide; tryptophan catabolic process; tryptophan catabolic process to kynurenine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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