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anti-NRXN1 antibody :: Goat Neurexin 1 Polyclonal Antibody

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Catalog # MBS421542
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Neurexin 1 (NRXN1), Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-Neurexin 1 Antibody

Product Synonym Names
neurexin 1; DKFZp313P2036; NRXN1; FLJ35941; Hs.22998; KIAA0578; neurexin I; neurexin 1 antibody; DKFZp313P2036 antibody; NRXN1 antibody; FLJ35941 antibody; Hs.22998 antibody; KIAA0578 antibody; neurexin I antibody; Neurexin 1; Neurexin 1
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KEKQPSSAKS SNKN
OMIM
600565
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KEKQPSSAKSSNKN, from the C Terminus of the protein sequence according to NP_004792.1 ; NP_620072.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both reported isoforms (NP_004792.1 and NP_620072.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-NRXN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NRXN1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NRXN1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx.75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1ug/ml.

Western Blot (WB) of anti-NRXN1 antibody
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
anti-NRXN1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NRXN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_620072.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,861 Da
NCBI Official Full Name
neurexin-1 isoform alpha1
NCBI Official Synonym Full Names
neurexin 1
NCBI Official Symbol
NRXN1  [Similar Products]
NCBI Official Synonym Symbols
PTHSL2; SCZD17; Hs.22998
  [Similar Products]
NCBI Protein Information
neurexin-1
UniProt Protein Name
Neurexin-1
UniProt Synonym Protein Names
Neurexin I-alpha; Neurexin-1-alpha
Protein Family
UniProt Gene Name
NRXN1  [Similar Products]
UniProt Synonym Gene Names
KIAA0578  [Similar Products]
UniProt Entry Name
NRX1A_HUMAN
NCBI Summary for NRXN1
This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission, and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. The RefSeq project has decided to create only a few representative transcript variants of the multitude that are possible. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2. [provided by RefSeq, Feb 2016]
UniProt Comments for NRXN1
NRXN1: Cell surface protein involved in cell-cell-interactions, excytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)- triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latroxin from spider venom. Belongs to the neurexin family. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cell soma; cell surface; endoplasmic reticulum; integral to plasma membrane; nuclear membrane; plasma membrane; presynaptic membrane; vesicle

Molecular Function: acetylcholine receptor binding; calcium channel regulator activity; calcium ion binding; cell adhesion molecule binding; protein binding; receptor activity

Biological Process: adult behavior; axon guidance; learning; neuromuscular process controlling balance; neuron adhesion; neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; social behavior; synaptic transmission; synaptogenesis; vocal learning

Disease: Chromosome 2p16.3 Deletion Syndrome; Pitt-hopkins-like Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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