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anti-PPY antibody :: Goat Pancreatic Polypeptide/PPY Polyclonal Antibody

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Catalog # MBS420040
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Pancreatic Polypeptide/PPY, Polyclonal Antibody

Popular Item
Also Known As

Goat anti-Pancreatic Polypeptide/PPY Antibody

Product Synonym Names
PPY; pancreatic polypeptide; HGNC: 9327; PNP; pancreatic polypeptide Y; PPY antibody; pancreatic polypeptide antibody; HGNC: 9327 antibody; PNP antibody; pancreatic polypeptide Y antibody; Pancreatic Polypeptide; Pancreatic Polypeptide / PPY
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
95
Immunogen Sequence
TRPRYGKRHK EDT
OMIM
167780
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PPY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PPY antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-PPY antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration, 3ug/ml. An anonymous customer found positive results in IHC on Mouse pancreas.
Western Blot: Preliminary experiments gave an approx 35-38kDa band in human pancreas lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.

Immunohistochemistry (IHC) of anti-PPY antibody
(3ug/ml) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
anti-PPY antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PPY. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
18,786 Da
NCBI Official Full Name
pancreatic prohormone isoform 1 preproprotein
NCBI Official Synonym Full Names
pancreatic polypeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
PP; PNP
  [Similar Products]
NCBI Protein Information
pancreatic prohormone
UniProt Protein Name
Pancreatic prohormone
UniProt Synonym Protein Names
Pancreatic polypeptide; PP
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PNP; PP; PH; PI  [Similar Products]
UniProt Entry Name
PAHO_HUMAN
NCBI Summary for PPY
This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Comments for PPY
PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding

Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; positive regulation of defense response to virus by host; protein secretion; regulation of appetite
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-PPY antibody
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