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anti-SOD1 antibody :: Goat SOD1 Polyclonal Antibody

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Catalog # MBS420750
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

SOD1, Polyclonal Antibody

Full Product Name

Goat anti-SOD1 Antibody

Product Synonym Names
Goat Anti-SOD1, Biotinylated Antibody; SOD1; superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)); HGNC: 11179; ALS; ALS1; IPOA; SOD; Cu /Zn superoxide dismutase; Cu/Zn superoxide dismutase; SOD, soluble; indophenoloxidase A; superoxide dismutase (aa 120-154); superoxide; SOD1 antibody; superoxide dismutase 1; soluble (amyotrophic lateral sclerosis 1 (adult)) antibody; HGNC: 11179 antibody; ALS antibody; ALS1 antibody; IPOA antibody; SOD antibody; Cu /Zn superoxide dismutase antibody; Cu/Zn superoxide dismutase antibody; SOD; soluble antibody; indophenoloxidase A antibody; superoxide dismutase (aa 120-154) antibody; superoxide dismutase 1; soluble antibody; superoxide dismutase; cystolic antibody
Matching Pairs
Unconjugated Antibody: SOD1 (MBS420750)
Biotin Conjugated Antibody: SOD1 (MBS423500)
Antibody/Peptide Pairs
SOD1 peptide (MBS426498) is used for blocking the activity of SOD1 antibody (MBS420750)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
SRKHGGPKDE ERH
OMIM
105400
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, etc
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SOD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SOD1 antibody
Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SOD1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, in purified membranes of cell line HeLa and in lysates of NIH3T3. Approx 20kDa observed in HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to human NP_000445.1, mouse NP_035564.1 and rat NP_058746.1). Recommended concentration: 0.01-0.03ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml.

Western Blot (WB) of anti-SOD1 antibody
(0. 02ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SOD1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-SOD1 antibody
(0. 01ug/ml) staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SOD1 antibody Western Blot (WB) (WB) image
Testing Data of anti-SOD1 antibody
(1.5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).
anti-SOD1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for SOD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,936 Da
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 1, soluble
NCBI Official Symbol
NCBI Official Synonym Symbols
ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
  [Similar Products]
NCBI Protein Information
superoxide dismutase [Cu-Zn]
UniProt Protein Name
Superoxide dismutase [Cu-Zn]
UniProt Synonym Protein Names
Superoxide dismutase 1; hSod1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
hSod1  [Similar Products]
UniProt Entry Name
SODC_HUMAN
NCBI Summary for SOD1
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SOD1
SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Apoptosis; Oxidoreductase; Nuclear receptor co-regulator; Mitochondrial; EC 1.15.1.1

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite cytoplasm; extracellular matrix; extracellular region; extracellular space; lysosome; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; myelin sheath; nucleoplasm; nucleus; peroxisome; plasma membrane; protein complex

Molecular Function: chaperone binding; copper ion binding; identical protein binding; protein binding; protein homodimerization activity; protein phosphatase 2B binding; Rac GTPase binding; superoxide dismutase activity; zinc ion binding

Biological Process: activation of MAPK activity; anterograde axon cargo transport; auditory receptor cell stereocilium organization and biogenesis; cell aging; cellular iron ion homeostasis; embryo implantation; glutathione metabolic process; heart contraction; hydrogen peroxide biosynthetic process; locomotory behavior; muscle maintenance; myelin maintenance in the peripheral nervous system; myeloid cell homeostasis; negative regulation of cholesterol biosynthetic process; negative regulation of neuron apoptosis; neurofilament cytoskeleton organization and biogenesis; ovarian follicle development; placenta development; platelet degranulation; positive regulation of apoptosis; positive regulation of catalytic activity; positive regulation of cytokine production; positive regulation of superoxide release; regulation of blood pressure; regulation of GTPase activity; regulation of mitochondrial membrane potential; regulation of multicellular organism growth; regulation of organ growth; regulation of protein kinase activity; regulation of T cell differentiation in the thymus; relaxation of vascular smooth muscle; removal of superoxide radicals; response to amphetamine; response to antibiotic; response to axon injury; response to copper ion; response to drug; response to ethanol; response to heat; response to hydrogen peroxide; response to nutrient levels; response to organic substance; response to reactive oxygen species; response to superoxide; retinal homeostasis; retrograde axon cargo transport; sensory perception of sound; spermatogenesis; superoxide metabolic process; superoxide release; thymus development; transmission of nerve impulse

Disease: Amyotrophic Lateral Sclerosis 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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