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anti-SORD antibody :: Goat Sorbitol Dehydrogenase Polyclonal Antibody

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Catalog # MBS420481
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Sorbitol Dehydrogenase (SORD), Polyclonal Antibody

Full Product Name

Goat anti-Sorbitol Dehydrogenase Antibody

Product Synonym Names
sorbitol dehydrogenase; SORD; SORD1; sorbitol dehydrogenase antibody; SORD antibody; SORD1 antibody; Sorbitol Dehydrogenase; Sorbitol Dehydrogenase
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KIMLKCDPSD QNP
OMIM
182500
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence KIMLKCDPSDQNP, from the C Terminus of the protein sequence according to NP_003095.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SORD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SORD antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SORD antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 38kDa band observed in Mouse Kidney lysates (calculated MW of 38.3kDa according to NP_003095.1). Recommended concentration: 0.1-0.3ug/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-SORD antibody
(0.1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SORD antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SORD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,812 Da
NCBI Official Full Name
sorbitol dehydrogenase
NCBI Official Synonym Full Names
sorbitol dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
SORD1; HEL-S-95n
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NCBI Protein Information
sorbitol dehydrogenase
UniProt Protein Name
Sorbitol dehydrogenase
UniProt Synonym Protein Names
L-iditol 2-dehydrogenase
Protein Family
UniProt Gene Name
UniProt Entry Name
DHSO_HUMAN
NCBI Summary for SORD
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
UniProt Comments for SORD
SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.14

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: cytosol; extracellular space; membrane; mitochondrial membrane

Molecular Function: carbohydrate binding; D-xylulose reductase activity; identical protein binding; L-iditol 2-dehydrogenase activity; NAD binding; zinc ion binding

Biological Process: fructose biosynthetic process; glucose metabolic process; glucuronate catabolic process to xylulose 5-phosphate; L-xylitol catabolic process; L-xylitol metabolic process; response to cadmium ion; response to copper ion; response to drug; response to hormone stimulus; response to nutrient levels; response to osmotic stress; sorbitol catabolic process; sperm motility
Product References and Citations for anti-SORD antibody
Cao W, Aghajanian HK, Haig-Ladewig LA, Gerton GL.; Sorbitol can fuel mouse sperm motility and protein tyrosine phosphorylation via sorbitol dehydrogenase.; Biol Reprod.2009 Jan;80(1): 124-33.; Pubmed 18799757

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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