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anti-WNT3 antibody :: Goat WNT3 Polyclonal Antibody

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Catalog # MBS421042
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

WNT3, Polyclonal Antibody

Also Known As

Goat anti-WNT3 Antibody

Product Synonym Names
WNT3; wingless-type MMTV integration site family, member 3; INT4; MGC131950; MGC138321; MGC138323; WNT-3 proto-oncogene protein precursor; WNT3 antibody; wingless-type MMTV integration site family; member 3 antibody; INT4 antibody; MGC131950 antibody; MGC138321 antibody; MGC138323 antibody; WNT-3 proto-oncogene protein precursor antibody
Antibody/Peptide Pairs
WNT3 peptide (MBS425179) is used for blocking the activity of WNT3 antibody (MBS421042)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
355
Immunogen Sequence
CGRGHNTRTE KRKEK
OMIM
165330
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-WNT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-WNT3 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-WNT3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml.
Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J.2011 Jul 11; PMID: 21746862.

Western Blot (WB) of anti-WNT3 antibody
(1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-WNT3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-WNT3 antibody
(2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-WNT3 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for WNT3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,645 Da
NCBI Official Full Name
proto-oncogene Wnt-3
NCBI Official Synonym Full Names
Wnt family member 3
NCBI Official Symbol
NCBI Official Synonym Symbols
INT4; TETAMS
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NCBI Protein Information
proto-oncogene Wnt-3
UniProt Protein Name
Proto-oncogene Wnt-3
UniProt Synonym Protein Names
Proto-oncogene Int-4 homolog
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
WNT3_HUMAN
NCBI Summary for WNT3
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]
UniProt Comments for WNT3
WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: frizzled binding; protein binding; protein domain specific binding; receptor agonist activity

Biological Process: anterior/posterior axis specification; axon guidance; cell fate commitment; cell morphogenesis; dorsal/ventral axis specification; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; gamete generation; limb bud formation; mesoderm formation; negative regulation of axon extension involved in axon guidance; neuron differentiation; positive regulation of collateral sprouting in the absence of injury; positive regulation of Wnt receptor signaling pathway; Spemann organizer formation at the anterior end of the primitive streak; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Disease: Tetraamelia, Autosomal Recessive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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