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anti-Gnao1 antibody :: Rabbit G PROTEIN ALPHA 0 Polyclonal Antibody

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Catalog # MBS220900
Unit / Price
  1 mL  /  $385 +1 FREE 8GB USB
Testing Data #1
Product Name

G PROTEIN ALPHA 0 (Gnao1), Polyclonal Antibody

Popular Item
Also Known As

RABBIT ANTI MOUSE G PROTEIN ALPHA 0

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
354
Immunogen Sequence
{C-}A-N-N- L-R-G-C-G- L-Y
3D Structure
ModBase 3D Structure for P18872
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Drosophila
N.B. Antibody reactivity and working conditions may vary between species.
Specificity
Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kDa forming a heterotrimeric protein complex with beta and Gamma subunits. Two isoforms of the ? subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 5.0 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Immunogen
Synthetic peptide corresponding to the amino acids 345 - 354 of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-Gnao1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Gnao1 antibody
Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kDa forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).
Applications Tested/Suitable for anti-Gnao1 antibody
ELISA (EIA), Western Blot (WB)*
Application Notes for anti-Gnao1 antibody
ELISA: Minimum Dilution: 1/500; Maximum Dilution: 1/1000;
Western Blotting: Application Note: This antibody detects band at 39 kDa when used under reducing conditions against rat brain.

Testing Data #1 of anti-Gnao1 antibody
Synthetic G Protein Alpha 0 detected with Rabbit anti Mouse G Protein Alpha 0 (MBS220900)
anti-Gnao1 antibody Testing Data #1 image
NCBI/Uniprot data below describe general gene information for Gnao1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,036 Da[Similar Products]
NCBI Official Full Name
guanine nucleotide-binding protein G(o) subunit alpha isoform B
NCBI Official Synonym Full Names
guanine nucleotide binding protein, alpha O
NCBI Official Symbol
Gnao1  [Similar Products]
NCBI Official Synonym Symbols
Gnao; alphaO; Galphao; AW050213
  [Similar Products]
NCBI Protein Information
guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding protein G(o) subunit alpha; GTP-binding protein alpha-o subunit; Go alpha
UniProt Protein Name
Guanine nucleotide-binding protein G(o) subunit alpha
UniProt Gene Name
Gnao1  [Similar Products]
UniProt Synonym Gene Names
Gna0; Gnao  [Similar Products]
UniProt Entry Name
GNAO_MOUSE
UniProt Comments for Gnao1
G-alpha(o): Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14. Interacts with RGS14. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, heterotrimeric alpha G((i/o/t/z)); G protein, heterotrimeric

Cellular Component: neuron projection; protein complex; membrane; dendrite; plasma membrane; heterotrimeric G-protein complex; intracellular; myelin sheath

Molecular Function: GTPase activity; corticotropin-releasing hormone receptor 1 binding; GTP binding; guanyl nucleotide binding; metal ion binding; nucleotide binding; GTPase activating protein binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; protein complex binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; negative regulation of calcium ion transport; metabolic process; dopamine receptor signaling pathway; locomotory behavior; signal transduction; response to organic nitrogen; cellular process; regulation of heart contraction; positive regulation of GTPase activity; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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