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anti-PLEC antibody :: Guinea Pig anti-Fish Plectin Polyclonal Antibody

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Catalog # MBS834370
Unit / Price
  0.1 mL  /  $475 +1 FREE 8GB USB
anti-PLEC antibody
Product Name

Plectin, Polyclonal Antibody

Popular Item
Also Known As

Plectin antibody

Product Synonym Names
Polyclonal Plectin; Anti Plectin; PLEC1; PLTN; PLEC1b; HD1; PCN; EBS1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
4684
OMIM
131950
3D Structure
ModBase 3D Structure for Q15149
Clonality
Polyclonal
Host
Guinea Pig
Species Reactivity
Fish
Specificity
Human Plectin C-terminal
Form/Format
Supplied in liquid form. Contains 0.09% sodium azide
Biological Significance
Plectin is a giant protein (c500 kDa) found in nearly all mammalian cells which acts as a link between the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. In addition plectin links the cytoskeleton to junctions found in the plasma membrane that structurally connect different cells.
Immunogen
Plectin antibody was raised in Guinea Pig using C-terminal "C" domain of recombinant human Plectin as the immunogen.
Preparation and Storage
Store at 2-8 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-PLEC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PLEC antibody
Guinea Pig polyclonal Plectin antibody
Product Categories/Family for anti-PLEC antibody
Applications Tested/Suitable for anti-PLEC antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PLEC antibody
IHC: 1:500
WB: 1:16,000
NCBI/Uniprot data below describe general gene information for PLEC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
514,775 Da[Similar Products]
NCBI Official Full Name
plectin
NCBI Official Synonym Full Names
plectin
NCBI Official Symbol
NCBI Official Synonym Symbols
HD1; PCN; EBS1; EBSO; PLTN; PLEC1; LGMD2Q; PLEC1b
  [Similar Products]
NCBI Protein Information
plectin
UniProt Protein Name
Plectin
UniProt Synonym Protein Names
Hemidesmosomal protein 1; HD1; Plectin-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PLEC1; PCN; PLTN; HD1  [Similar Products]
UniProt Entry Name
PLEC_HUMAN
NCBI Summary for PLEC
Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (reviewed in PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used. [provided by RefSeq, Aug 2011]
UniProt Comments for PLEC
Plectin-1: a major cytoskeleton crosslinking protein that binds to actin, intermediate filaments and microtubules. A scaffold for proteins involved in cellular signaling. Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the cross-linking and stabilization of cytoskeletal intermediate filaments network, but also in the regulation of their dynamics. Regulates the organization of keratin intermediate filaments (IF) and MAPK signaling during cell migration. Downregulates PKC signaling by sequestering RACK1. Redistributes to the uropod associated with vimentin and fodrin during polarization of peripheral blood T lymphocytes. This vimentin-plectin-fodrin complex provides a continuous linkage from the nucleus (lamin B) to the cortical cytoskeleton. Defects in PLEC1 are the cause of epidermolysis bullosa simplex 1 (EBS1). Three alternatively spliced isoforms have been described.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: intermediate filament cytoskeleton; focal adhesion; costamere; hemidesmosome; cytoplasm; plasma membrane; sarcoplasm; cytosol; sarcolemma

Molecular Function: protein binding; structural constituent of muscle; ankyrin binding; actin binding

Biological Process: extracellular matrix organization and biogenesis; hemidesmosome assembly; apoptosis; cell structure disassembly during apoptosis
Precautions
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Disclaimer
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