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anti-XRCC5 antibody :: Goat XRCC5 / Ku80 Polyclonal Antibody

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Catalog # MBS242117
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

XRCC5 / Ku80, Polyclonal Antibody

Also Known As

Goat Polyclonal to Human XRCC5 / Ku80

Product Synonym Names
Anti-XRCC5 / Ku80 Antibody (Internal) IHC-plus; XRCC5; 86 kDa subunit of Ku antigen; CTC85; CTCBF; DNA repair protein XRCC5; G22P2; KARP-1; Ku autoantigen; 80kDa; NFIV; KARP1; Ku86; KUB2; TLAA; Nuclear factor IV; KU80; Thyroid-lupus autoantigen; Human XRCC5; Ku80
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
732
OMIM
194364
3D Structure
ModBase 3D Structure for P13010
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Orangutan
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Specificity
Human XRCC5 / Ku80.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Target Species
Human
Immunogen Description
Synthetic peptide C-SLAKKDEKTDTLED from an internal region of human XRCC5 / Ku80 (NP_066964.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (93%).
Immunogen Type
Synthetic peptide
Immunogen
XRCC5 / Ku80 antibody was raised against synthetic peptide C-SLAKKDEKTDTLED from an internal region of human XRCC5 / Ku80 (NP_066964.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (93%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-XRCC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-XRCC5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-XRCC5 antibody
ELISA (1:16000), IHC-P (2.5 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-XRCC5 antibody
Anti-XRCC5 / Ku80 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-XRCC5 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for XRCC5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
82,705 Da
NCBI Official Full Name
X-ray repair cross-complementing protein 5
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
NCBI Official Symbol
XRCC5  [Similar Products]
NCBI Official Synonym Symbols
KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
  [Similar Products]
NCBI Protein Information
X-ray repair cross-complementing protein 5; TLAA; CTC85; CTCBF; nuclear factor IV; Ku autoantigen, 80kDa; DNA repair protein XRCC5; thyroid-lupus autoantigen; 86 kDa subunit of Ku antigen; lupus Ku autoantigen protein p86; Ku86 autoantigen related protein 1; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit
UniProt Protein Name
X-ray repair cross-complementing protein 5
UniProt Synonym Protein Names
86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
UniProt Gene Name
XRCC5  [Similar Products]
UniProt Synonym Gene Names
G22P2; CTC85; CTCBF; TLAA  [Similar Products]
UniProt Entry Name
XRCC5_HUMAN
NCBI Summary for XRCC5
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
UniProt Comments for XRCC5
Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: RNA-binding; Helicase; Nuclear receptor co-regulator; EC 3.6.1.-; EC 3.6.4.-; DNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; nuclear telomere cap complex; membrane; nuclear chromosome, telomeric region; nucleolus; plasma membrane; cytosol; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; telomeric DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: response to drug; positive regulation of neurogenesis; transcription, DNA-dependent; viral reproduction; DNA repair; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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