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anti-ACAN antibody :: Rabbit Aggrecan Polyclonal Antibody

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Catalog # MBS243450
Unit / Price
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  0.05 mg  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Aggrecan (ACAN), Polyclonal Antibody

Full Product Name

Rabbit Polyclonal to Human Aggrecan

Product Synonym Names
Anti-Aggrecan Antibody (C-Terminus) IHC-plus; ACAN; AGCAN; Aggrecan core protein; Acg1; Aggrecan; CSPG1; CSPGCP; CSPCP; AGC1; Aggrecan 1; Large aggregating proteoglycan; MSK16; SEDK; MCSPG; Human Aggrecan
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 612813
3D Structure
ModBase 3D Structure for P16112
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Predicted Reactivity: Gorilla, Monkey (at least 90% immunogen sequence identity)
Specificity
Human ACAN / Aggrecan. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FAM59A (56%).
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.1% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic 18 amino acid peptide from C-terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Monkey (94%); Marmoset (83%).
Immunogen Type
Synthetic peptide
Immunogen
Aggrecan antibody was raised against synthetic 18 amino acid peptide from C-terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Monkey (94%); Marmoset (83%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.
Other Notes
Small volumes of anti-ACAN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACAN antibody
Aggrecan Core Protein, ESTs have been isolated from lung, uterus, brain, colon, bone, adrenal, stomach, breast, liver/spleen, fetal lung, fetal testis, and fetal B-cell libraries.
Applications Tested/Suitable for anti-ACAN antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-ACAN antibody
IHC-P (3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-ACAN antibody
Anti-ACAN / Aggrecan antibody IHC of human cartilage. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
anti-ACAN antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ACAN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
239,235 Da
NCBI Official Full Name
aggrecan core protein isoform 2
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
NCBI Official Synonym Symbols
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
  [Similar Products]
NCBI Protein Information
aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
UniProt Protein Name
Aggrecan core protein
UniProt Synonym Protein Names
Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1  [Similar Products]
UniProt Entry Name
PGCA_HUMAN
NCBI Summary for ACAN
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ACAN
ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Extracellular matrix

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region

Molecular Function: protein binding; metal ion binding; extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: keratan sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; keratan sulfate catabolic process; proteolysis; cell adhesion; skeletal development

Disease: Spondyloepiphyseal Dysplasia, Kimberley Type; Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type
Precautions
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Disclaimer
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