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anti-NKX2-5 antibody :: Goat NKX2-5 Polyclonal Antibody

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Catalog # MBS248997
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Product Name

NKX2-5, Polyclonal Antibody

Popular Item
Also Known As

Goat Polyclonal to Human NKX2-5

Product Synonym Names
Anti-NKX2-5 Antibody (Internal) IHC-plus; NKX2-5; Cardiac-specific homeo box; Cardiac-specific homeobox 1; Cardiac-specific homeobox; CSX1; CHNG5; HLHS2; NKX2E; NKX4-1; NKX2.5; Tinman paralog; VSD3; CSX; Homeobox protein CSX; Homeobox protein Nkx-2.5; NK2 homeobox 5; Human NKX2-5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
324
OMIM
phenotype 614435
3D Structure
ModBase 3D Structure for P52952
Clonality
Polyclonal
Host
Goat
Species Reactivity
Reactivity: Human, Monkey (tested or 100% immunogen sequence identity)
Predicted: Rat, Hamster, Pig (at least 90% immunogen sequence identity)
Specificity
Human NKX2-5. This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide C-PRAYSDPDPAKDPR from an internal region of human NKX2-5 (NP_004378.1; NP_001159647.1; NP_001159648.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Rat, Hamster, Elephant, Panda, Pig (93%).
Immunogen Type
Synthetic peptide
Immunogen
NKX2-5 antibody was raised against synthetic peptide C-PRAYSDPDPAKDPR from an internal region of human NKX2-5 (NP_004378.1; NP_001159647.1; NP_001159648.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Rat, Hamster, Elephant, Panda, Pig (93%); Bovine, Dog (86%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-NKX2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NKX2-5 antibody
Nkx 2.5 is a lineage restricted transcription factor that regulates the expression of heart specific genes. This regulation is essential for tissue differentiation and the determination of temporal and spatial patterns of development. Abundant expression of Nkx 2.5 is observed in the mouse heart from the time of cardiac differentiation through adult life. Targeted disruption of Nkx 2.5 in mice results in early embryonic lethality with cardiac development arrested at the linear tube stage, prior to looping.
Applications Tested/Suitable for anti-NKX2-5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-NKX2-5 antibody
Usage Summary: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kDa band observed in Human Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.3-1 ug/ml.

Uses: IHC - Paraffin (3.75 µg/ml), Western blot (0.3 - 1 µg/ml), ELISA (1:32000)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-NKX2-5 antibody
Anti-NKX2-5 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
anti-NKX2-5 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-NKX2-5 antibody
NKX2-5 antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-NKX2-5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NKX2-5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
homeobox protein Nkx-2.5 isoform 1
NCBI Official Synonym Full Names
NK2 homeobox 5
NCBI Official Symbol
NKX2-5  [Similar Products]
NCBI Official Synonym Symbols
CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1
  [Similar Products]
NCBI Protein Information
homeobox protein Nkx-2.5; tinman paralog; homeobox protein CSX; cardiac-specific homeobox 1; homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5
UniProt Protein Name
Homeobox protein Nkx-2.5
UniProt Synonym Protein Names
Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
Protein Family
UniProt Gene Name
NKX2-5  [Similar Products]
UniProt Synonym Gene Names
CSX; NKX2.5; NKX2E  [Similar Products]
UniProt Entry Name
NKX25_HUMAN
NCBI Summary for NKX2-5
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Comments for NKX2-5
NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; ventricular cardiac myofibril development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; atrial cardiac muscle cell development; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; regulation of cardiac muscle cell proliferation; hemopoiesis; heart looping; vasculogenesis; cell differentiation; cardiac muscle contraction; spleen development; adult heart development; pharyngeal system development; sarcomere organization; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; positive regulation of heart contraction; negative regulation of apoptosis

Disease: Ventricular Septal Defect 3; Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Tetralogy Of Fallot; Hypothyroidism, Congenital, Nongoitrous, 5; Hypoplastic Left Heart Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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