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anti-Ccnb1 antibody :: Rabbit M CCNB1 Polyclonal Antibody

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Catalog # MBS9211743
Unit / Price
Scan QR to view Datasheet
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

M CCNB1, Polyclonal Antibody

Full Product Name

M CCNB1 Antibody (Center S123/S125)

Product Synonym Names
G2/mitotic-specific cyclin-B1; Ccnb1; Ccn-2; Ccnb1-rs13; Cycb; Cycb1
Antibody/Peptide Pairs
M CCNB1 peptide (MBS9229050) is used for blocking the activity of M CCNB1 antibody (MBS9211743)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
103-130
3D Structure
ModBase 3D Structure for P24860
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Mouse (Predicted Reactivity: Hamster)
Specificity
This Mouse CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the Central region of mouse CCNB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.32 (lot specific)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-Ccnb1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Ccnb1 antibody
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Applications Tested/Suitable for anti-Ccnb1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-Ccnb1 antibody
IHC~~1:25
WB~~1:1000

Immunohistochemistry (IHC) of anti-Ccnb1 antibody
Immunohistochemical analysis of paraffin-embedded M. testis section using M CCNB1 Antibody (Center S123/S125). MBS9211743 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
anti-Ccnb1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-Ccnb1 antibody
Immunohistochemical analysis of paraffin-embedded M. spleen section using M CCNB1 Antibody (Center S123/S125). MBS9211743 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
anti-Ccnb1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-Ccnb1 antibody
Mouse CCNB1 Antibody (Center S123/S125) western blot analysis in mouse NIH/3T3 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow).
anti-Ccnb1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for Ccnb1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
48052
NCBI Official Full Name
G2/mitotic-specific cyclin-B1
NCBI Official Synonym Full Names
cyclin B1
NCBI Official Symbol
Ccnb1  [Similar Products]
NCBI Official Synonym Symbols
CycB1; Cycb-4; Cycb-5; Ccnb1-rs1; Cycb1-rs1; Ccnb1-rs13
  [Similar Products]
NCBI Protein Information
G2/mitotic-specific cyclin-B1
UniProt Protein Name
G2/mitotic-specific cyclin-B1
UniProt Gene Name
Ccnb1  [Similar Products]
UniProt Synonym Gene Names
Ccn-2; Ccnb1-rs13; Cycb; Cycb1  [Similar Products]
UniProt Entry Name
CCNB1_MOUSE
UniProt Comments for Ccnb1
CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Cellular Component: spindle pole; centrosome; cytoskeleton; membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein complex binding; histone kinase activity; patched binding; kinase activity; protein kinase binding

Biological Process: mitosis; positive regulation of histone phosphorylation; in utero embryonic development; regulation of cell cycle; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of mRNA 3'-end processing; positive regulation of cell cycle; cell cycle; positive regulation of mitotic cell cycle; mitotic metaphase plate congression; protein amino acid phosphorylation; oocyte maturation; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; cell division; protein complex assembly; regulation of cyclin-dependent protein kinase activity; mitotic spindle stabilization; positive regulation of cardiac muscle cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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