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anti-IGFBP3 antibody :: Rabbit Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Polyclonal Antibody

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Catalog # MBS2005953 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.1 mg  /  $170 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Insulin Like Growth Factor Binding Protein 3 (IGFBP3), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
291
Chromosome Location
Chromosome: 4; AC_000161.1 (76705105..76713511). Location: chromosome: 4
3D Structure
ModBase 3D Structure for P20959
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Homo sapiens (Human)
Specificity
The antibody is a rabbit polyclonal antibody raised against IGFBP3. It has been selected for its ability to recognize IGFBP3 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-IGFBP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IGFBP3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-IGFBP3 antibody
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-IGFBP3 antibody
Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human IGFBP3 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(#MBS2086047)
anti-IGFBP3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for IGFBP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,570 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 3<
NCBI Official Symbol
IGFBP3  [Similar Products]
NCBI Official Synonym Symbols
IGFBP-3
  [Similar Products]
NCBI Protein Information
insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; insulin-like growth factor binding protein-3
UniProt Protein Name
Insulin-like growth factor-binding protein 3
UniProt Gene Name
IGFBP3  [Similar Products]
UniProt Synonym Gene Names
IBP-3; IGF-binding protein 3; IGFBP-3  [Similar Products]
UniProt Entry Name
IBP3_BOVIN
UniProt Comments for IGFBP3
Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Subunit structure: Interacts with HN. Interacts with TMEM219

By similarity. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with XLKD1. Ref.6

Subcellular location: Secreted.

Tissue specificity: Plasma; expressed by most tissues.

Post-translational modification: Phosphorylation sites are present in the extracellular medium

By similarity.

Sequence similarities: Contains 1 IGFBP N-terminal domain.Contains 1 thyroglobulin type-1 domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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