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Goat anti Horse IgM (Fc specific) Secondary Antibody

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Catalog # MBS571482
Unit / Price
  1 mL  /  $290 +1 FREE 8GB USB
Goat anti Horse IgM (Fc specific) secondary antibody
Product Name

Goat anti Horse IgM (Fc specific), Secondary Antibody

Popular Item
Also Known As

Goat anti Horse IgM (Fc specific)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Host
Goat
Species Reactivity
The antiserum does not cross-react with any other component of the horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivit
Specificity
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal horse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments.
Form/Format
Delipidated, heat inactivated, lyophilized, stable whole antiserum
Preservative
No preservative added
Immunogen
Highly purified normal IgM isolated from pooled horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Absorption
Immunoaffinity adsorbed using in solubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevent the presence of excess adsorbent protein or immune complexes in the antiserum.
Protein Concentrion
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Antibody Titre
Precipitin titre 1 :32 when tested against pooled normal horse serum in agar-block immunodiffusion titration.
Direction of use
In immunoelectrophoresis use 2 uL or equivalent against 120 uL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 uL in a 3 mm diameter center well and 2 uL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivity Note
The antiSerum does not cross-react with any other component of the Horse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Packing:Vial with 1 ml lyophilized antiserum.

Lyophilized at +4 degree C = at least 10 years
reconstituted at or below -20 degree C =3-5 years
reconstituted at +4 degree C = 7 days

Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
Other Notes
Small volumes of Goat anti Horse IgM (Fc specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma.
Immunoprecipitation (IP)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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