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anti-S17aH antibody :: Rabbit anti-Human 17-Alpha-Hydroxylase Polyclonal Antibody

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Catalog # MBS2032266 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

17-Alpha-Hydroxylase (S17aH), Polyclonal Antibody

Full Product Name

17-Alpha-Hydroxylase (S17aH) Polyclonal Antibody

Product Synonym Names
CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17, 20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450, Family 17, Subfamily A, Polypeptide 1
Matching Pairs
Matching Pairs
Unconjugated Antibody: 17-Alpha-Hydroxylase (MBS2032266)
APC-CY7 Conjugated Antibody: 17-Alpha-Hydroxylase (S17aH) (MBS2062095)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M14564 mRNA
3D Structure
ModBase 3D Structure for P05093
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against S17aH. It has been selected for its ability to recognize S17aH in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Human
Fragment
S17aH (Cys183~Thr508)
Quality Control
Content: The quality control contains recombinant S17aH (Cys183~Thr508) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062097
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-S17aH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-S17aH antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-S17aH antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-S17aH antibody
Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Jurkat Cells.
anti-S17aH antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-S17aH antibody
Western Blot: Sample: Recombinant S17aH, Human.
anti-S17aH antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-S17aH antibody
DAB staining on fromalin fixed paraffin- embedded adrenal gland tissue)
anti-S17aH antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for S17aH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,371 Da
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450 family 17 subfamily A member 1
NCBI Official Symbol
CYP17A1  [Similar Products]
NCBI Official Synonym Symbols
CPT7; CYP17; S17AH; P450C17
  [Similar Products]
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Synonym Protein Names
17-alpha-hydroxyprogesterone aldolase (EC:1.14.14.32
UniProt Gene Name
CYP17A1  [Similar Products]
UniProt Synonym Gene Names
CYP17; S17AH; Cytochrome P450c17  [Similar Products]
NCBI Summary for S17aH
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
UniProt Comments for S17aH
CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: EC 1.14.99.9; EC 4.1.2.30; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: axon; cell soma; endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrion

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; heme binding; iron ion binding; oxygen binding; steroid 17-alpha-monooxygenase activity

Biological Process: androgen biosynthetic process; glucocorticoid biosynthetic process; hormone biosynthetic process; progesterone metabolic process; sex differentiation; steroid biosynthetic process; steroid metabolic process; sterol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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