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anti-HIST1H1C antibody :: Rabbit anti-Human 2-hydroxyisobutyryl-HIST1H1C Polyclonal Antibody

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Catalog # MBS7110685
Unit / Price
  0.05 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $250 +1 FREE 8GB USB
Product Name

2-hydroxyisobutyryl-HIST1H1C, Polyclonal Antibody

Full Product Name

2-hydroxyisobutyryl-HIST1H1C(K25) Antibody

Product Synonym Names
Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1C; H1F2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X57129 Genomic DNA
3D Structure
ModBase 3D Structure for P16403
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Immunogen
Peptide sequence around site of 2-hydroxyisobutyryl-Lys(25) derived from human Histone H1.2
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HIST1H1C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HIST1H1C antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-HIST1H1C antibody
IHC: 1:20-1:200
IF: 1:50-1:200

Immunocytochemistry (ICC) of anti-HIST1H1C antibody
Immunocytochemistry analysis of MBS7110685 diluted at 1:10 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
anti-HIST1H1C antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-HIST1H1C antibody
Immunofluorescence staining of Hela cells(treated with 30mM sodium butyrate for 4h) with MBS7110685 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
anti-HIST1H1C antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for HIST1H1C. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,365 Da
NCBI Official Full Name
histone H1.2
NCBI Official Synonym Full Names
histone cluster 1 H1 family member c
NCBI Official Symbol
HIST1H1C  [Similar Products]
NCBI Official Synonym Symbols
H1C; H1.2; H1F2; H1s-1
  [Similar Products]
NCBI Protein Information
histone H1.2
UniProt Protein Name
Histone H1.2
UniProt Synonym Protein Names
Histone H1c; Histone H1d; Histone H1s-1
UniProt Gene Name
HIST1H1C  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for HIST1H1C
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
UniProt Comments for HIST1H1C
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-HIST1H1C antibody
 Reference Product  PubMed Publications
 CDK1 antibody  >1 publications with HIST1H1C and CDK1
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