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anti-RPS18 antibody :: Rabbit anti-Human 40S ribosomal protein S18 Polyclonal Antibody

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Catalog # MBS715962
Unit / Price
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  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Western Blot (WB)
Product Name

40S ribosomal protein S18 (RPS18), Polyclonal Antibody

Full Product Name

Rabbit anti-human 40S ribosomal protein S18 polyclonal Antibody, Biotin conjugated

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
180473
3D Structure
ModBase 3D Structure for P62269
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Biotin
Immunogen
Recombinant human 40S ribosomal protein S18 protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-161198 / sc-161199
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-RPS18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RPS18 antibody
Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA
Applications Tested/Suitable for anti-RPS18 antibody
ELISA (EIA)

Western Blot (WB) of anti-RPS18 antibody
Western blot 40S ribosomal protein S18 Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 16 kDaObserved band size: 75 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
anti-RPS18 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for RPS18. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,719 Da[Similar Products]
NCBI Official Full Name
40S ribosomal protein S18
NCBI Official Synonym Full Names
ribosomal protein S18
NCBI Official Symbol
RPS18  [Similar Products]
NCBI Official Synonym Symbols
KE3; S18; HKE3; KE-3; D6S218E
  [Similar Products]
NCBI Protein Information
40S ribosomal protein S18
UniProt Protein Name
40S ribosomal protein S18
UniProt Synonym Protein Names
Ke-3; Ke3
Protein Family
UniProt Gene Name
RPS18  [Similar Products]
UniProt Synonym Gene Names
D6S218E; Ke3  [Similar Products]
UniProt Entry Name
RS18_HUMAN
NCBI Summary for RPS18
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Comments for RPS18
RPS18: Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA. Belongs to the ribosomal protein S13P family.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytosol; focal adhesion; membrane; nucleus; ribosome; small ribosomal subunit

Molecular Function: protein binding; rRNA binding; structural constituent of ribosome

Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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