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anti-53BP1 antibody :: Rabbit anti-Human 53BP1 Polyclonal Antibody

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Catalog # MBS9409587
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

53BP1, Polyclonal Antibody

Also Known As

53BP1 (Ab-29) Antibody

Product Synonym Names
p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1975
OMIM
605230
3D Structure
ModBase 3D Structure for Q12888
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody detects endogenous levels of total 53BP1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from Internal of human 53BP1.
Target Name
53BP1
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-53BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-53BP1 antibody
Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation.Iwabuchi K., J. Biol. Chem. 273:26061-26068(1998).Nakajima D., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.Bechtel S., BMC Genomics 8:399-399(2007).
Product Categories/Family for anti-53BP1 antibody
Applications Tested/Suitable for anti-53BP1 antibody
Western Blot (WB)
Application Notes for anti-53BP1 antibody
Western blotting: 1:500~1:3000

Western Blot (WB) of anti-53BP1 antibody
Western blot analysis of extracts from Jurkat cells and HUVEC cells, using 53BP1 (Ab-29) antibody.
anti-53BP1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-53BP1 antibody
Western blot analysis of extracts from 293 cells (Lane 2), using 53BP1 (Ab-29) antiobdy. The lane on the left is treated with synthesized peptide.
anti-53BP1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for 53BP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
213,989 Da
NCBI Official Full Name
tumor suppressor p53-binding protein 1 isoform 2
NCBI Official Synonym Full Names
tumor protein p53 binding protein 1
NCBI Official Symbol
TP53BP1  [Similar Products]
NCBI Official Synonym Symbols
p202; 53BP1; p53BP1
  [Similar Products]
NCBI Protein Information
tumor suppressor p53-binding protein 1
UniProt Protein Name
Tumor suppressor p53-binding protein 1
UniProt Gene Name
TP53BP1  [Similar Products]
UniProt Synonym Gene Names
53BP1; p53-binding protein 1; p53BP1  [Similar Products]
UniProt Entry Name
TP53B_HUMAN
UniProt Comments for 53BP1
53BP1: a DNA damage checkpoint protein that interacts with HDAC4 protein to mediate the DNA damage response. Inhibits E2F1-mediated apoptosis in prostate cancer cells. Recruited to double strand breaks via the binding of its Tudor domain to dimethylated H3 K20. Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Binds to the central domain of p53. Interacts with Artemis. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on S139. Interacts with histone H4 that has been dimethylated at K20. Has low affinity for histone H4 containing monomethylated K20. Does not bind histone H4 containing unmethylated or trimethylated K20. Has low affinity for histone H3 that has been dimethylated on K79. Does not bind unmethylated histone H3. Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Two alternatively spliced human isoforms have been observed.

Protein type: Transcription, coactivator/corepressor; DNA repair, damage

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasm; replication fork; nucleus

Molecular Function: protein binding; p53 binding; damaged DNA binding; telomeric DNA binding; methylated histone residue binding

Biological Process: transcription from RNA polymerase II promoter; double-strand break repair; positive regulation of transcription, DNA-dependent; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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