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anti-ADAM10 antibody :: Rabbit anti-Human A Disintegrin And Metalloprotease 10 (ADAM10) Polyclonal Antibody

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Catalog # MBS2002720 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $165 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $310 +1 FREE 8GB USB
  1 mg  /  $725 +1 FREE 8GB USB
Product Name

A Disintegrin And Metalloprotease 10 (ADAM10), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- R ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN CFVESGQPIC GNGMVEQGEE CDCGYSDQCK DECCFDANQP EGRKCKLKPG KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT ALCPASDPKP NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD V
OMIM
AF009615 mRNA
3D Structure
ModBase 3D Structure for O14672
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against ADAM10. It has been selected for its ability to recognize ADAM10 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant ADAM10 (Arg420~Val641) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047073
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ADAM10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ADAM10 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-ADAM10 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-ADAM10 antibody
Western Blot: Sample: Human Placenta Tissue.
anti-ADAM10 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ADAM10 antibody
Western Blot: Sample: Recombinant ADAM10, Human.
anti-ADAM10 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ADAM10 antibody
HE staining on IHC-P; Samples: Human Kidney Tissue.
anti-ADAM10 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ADAM10 antibody
HE staining on IHC-P; Samples: Human Liver Tissue
anti-ADAM10 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ADAM10 antibody
DAB staining on IHC-P; Samples: Human Kidney Tissue
anti-ADAM10 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ADAM10 antibody
DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue
anti-ADAM10 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ADAM10 antibody
DAB staining on IHC-P; Samples: Human Stomach Tissue.
anti-ADAM10 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ADAM10. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,047 Da
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 10 isoform 1 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
ADAM10  [Similar Products]
NCBI Official Synonym Symbols
RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Synonym Protein Names
CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD_antigen: CD156c
UniProt Gene Name
ADAM10  [Similar Products]
UniProt Synonym Gene Names
KUZ; MADM; ADAM 10  [Similar Products]
NCBI Summary for ADAM10
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]
UniProt Comments for ADAM10
ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Cell adhesion; Cell surface; EC 3.4.24.81; Membrane protein, integral; Motility/polarity/chemotaxis; Protease; Vesicle

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular exosome; focal adhesion; Golgi apparatus; Golgi membrane; Golgi-associated vesicle; integral component of membrane; membrane; nucleus; plasma membrane; postsynaptic density

Molecular Function: endopeptidase activity; integrin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; protein homodimerization activity; protein kinase binding; receptor binding; SH3 domain binding

Biological Process: cell-cell signaling; cellular protein metabolic process; constitutive protein ectodomain proteolysis; extracellular matrix disassembly; in utero embryonic development; integrin-mediated signaling pathway; membrane protein ectodomain proteolysis; monocyte activation; negative regulation of cell adhesion; neutrophil degranulation; Notch receptor processing; Notch signaling pathway; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of T cell chemotaxis; post-translational protein modification; protein amino acid phosphorylation; protein processing; response to tumor necrosis factor

Disease: Alzheimer Disease 18; Reticulate Acropigmentation Of Kitamura
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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