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anti-ADAM12 antibody :: Rabbit anti-Human A Disintegrin And Metalloprotease 12 Polyclonal Antibody

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Catalog # MBS2028685 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $90 +1 FREE 8GB USB
  0.02 mg  /  $105 +1 FREE 8GB USB
  0.05 mg  /  $135 +1 FREE 8GB USB
  0.1 mg  /  $170 +1 FREE 8GB USB
  0.2 mg  /  $235 +1 FREE 8GB USB
  1 mg  /  $540 +1 FREE 8GB USB
Product Name

A Disintegrin And Metalloprotease 12 (ADAM12), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to A Disintegrin And Metalloprotease 12 (ADAM12)

Matching Pairs
Unconjugated Antibody: A Disintegrin And Metalloprotease 12 (MBS2028685)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: A Disintegrin And Metalloprotease 12 (MBS2028685)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
19
OMIM
AF023476 mRNA
3D Structure
ModBase 3D Structure for O43184
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against ADAM12. It has beenselected for its ability to recognize ADAM12 in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant ADAM12 (Ser513~His767) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2082140
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of anti-ADAM12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ADAM12 antibody
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
Application Notes for anti-ADAM12 antibody
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ADAM12 antibody
Western Blot: Sample: Recombinant protein.
anti-ADAM12 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ADAM12 antibody
DABstainingonIHC-P.Samples:HumanTissue)
anti-ADAM12 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ADAM12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,263 Da
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 12 isoform 4 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 12
NCBI Official Symbol
ADAM12  [Similar Products]
NCBI Official Synonym Symbols
MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 12
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 12
UniProt Synonym Protein Names
Meltrin-alpha
UniProt Gene Name
ADAM12  [Similar Products]
UniProt Synonym Gene Names
MLTN; ADAM 12  [Similar Products]
NCBI Summary for ADAM12
This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
UniProt Comments for ADAM12
ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.

Protein type: EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: extracellular space; integral component of membrane; nucleoplasm; plasma membrane

Molecular Function: metal ion binding; metalloendopeptidase activity; metallopeptidase activity; SH3 domain binding

Biological Process: cell adhesion; extracellular matrix organization; myoblast fusion; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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