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anti-AADAT antibody :: Goat AADAT Polyclonal Antibody

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Catalog # MBS421820
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

AADAT, Polyclonal Antibody

Also Known As

Goat anti-AADAT Antibody

Product Synonym Names
Goat Anti-AADAT (int. ) Antibody; AADAT; aminoadipate aminotransferase; KAT2; KATII; L kynurenine/alpha aminoadipate aminotransferase; L-kynurenine/alpha-aminoadipate aminotransferase; alpha-aminoadipate aminotransferase; kynurenine aminotransferase II; AADAT antibody; aminoadipate aminotransferase antibody; KAT2 antibody; KATII antibody; L kynurenine/alpha aminoadipate aminotransferase antibody; L-kynurenine/alpha-aminoadipate aminotransferase antibody; alpha-aminoadipate aminotransferase antibody; kynurenine aminotransferase II antibody
Antibody/Peptide Pairs
AADAT peptide (MBS426425) is used for blocking the activity of AADAT antibody (MBS421820)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
425
Immunogen Sequence
KPEDAKNPQK NTPK
OMIM
611754
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KPEDAKNPQKNTPK, from the internal region of the protein sequence according to NP_057312.1; NP_872603.1.
Epitope
Internal region
Note
Both reported variants represent identical protein (NP_057312.1and NP_872603.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-AADAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AADAT antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-AADAT antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 45kDa band observed in Human Liver lysates (calculated MW of 47.4kDa according to NP_057312.1). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-AADAT antibody
(1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-AADAT antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AADAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_872603.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,822 Da
NCBI Official Full Name
kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform b
NCBI Official Synonym Full Names
aminoadipate aminotransferase
NCBI Official Symbol
AADAT  [Similar Products]
NCBI Official Synonym Symbols
KAT2; KATII; KYAT2
  [Similar Products]
NCBI Protein Information
kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
UniProt Protein Name
Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
UniProt Synonym Protein Names
2-aminoadipate aminotransferase; 2-aminoadipate transaminase (EC:2.6.1.39); Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2 (EC:2.6.1.7); Kynurenine--oxoglutarate transaminase II
UniProt Gene Name
AADAT  [Similar Products]
UniProt Synonym Gene Names
KAT2; KAT/AadAT; AadAT  [Similar Products]
UniProt Entry Name
AADAT_HUMAN
NCBI Summary for AADAT
This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Comments for AADAT
AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.6.1.39; Amino Acid Metabolism - tryptophan; EC 2.6.1.7; Transferase; Mitochondrial; Amino Acid Metabolism - lysine biosynthesis

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: mitochondrial matrix

Molecular Function: 2-aminoadipate transaminase activity; kynurenine-oxoglutarate transaminase activity; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: 2-oxoglutarate metabolic process; biosynthetic process; glutamate metabolic process; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process; tryptophan catabolic process; tryptophan catabolic process to kynurenine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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