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anti-AARS2 antibody :: Rabbit anti-Human AARS2 Polyclonal Antibody

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Catalog # MBS9207929
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

AARS2, Polyclonal Antibody

Also Known As

AARS2 Antibody (Center)

Product Synonym Names
Alanine--tRNA ligase; mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133}; 6117 {ECO:0000255|HAMAP-Rule:MF_03133}; Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}; AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}; AARS2 {ECO:0000255|HAMAP-Rule:MF_03133}
Antibody/Peptide Pairs
AARS2 peptide (MBS9226998) is used for blocking the activity of AARS2 antibody (MBS9207929)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
366-394
Immunogen Sequence Length
985
OMIM
612035
3D Structure
ModBase 3D Structure for Q5JTZ9
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Specificity
This AARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the Central region of human AARS2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-AARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AARS2 antibody
Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction:

ATP + L-alanine + tRNAAla
<---> AMP + diphosphate + L-alanyl-tRNAAla.



This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
Applications Tested/Suitable for anti-AARS2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-AARS2 antibody
WB~~1:1000

Western Blot (WB) of anti-AARS2 antibody
AARS2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the AARS2 antibody detected the AARS2 protein (arrow).
anti-AARS2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AARS2 antibody
AARS2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AARS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-AARS2 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-AARS2 antibody
AARS2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-AARS2 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for AARS2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
107340
NCBI Official Full Name
alanine--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
alanyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
AARS2  [Similar Products]
NCBI Official Synonym Symbols
AARSL; LKENP; COXPD8; MTALARS; MT-ALARS
  [Similar Products]
NCBI Protein Information
alanine--tRNA ligase, mitochondrial
UniProt Protein Name
Alanine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Alanyl-tRNA synthetase
UniProt Gene Name
AARS2  [Similar Products]
UniProt Synonym Gene Names
AlaRS  [Similar Products]
UniProt Entry Name
SYAM_HUMAN
NCBI Summary for AARS2
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8. [provided by RefSeq, Dec 2011]
UniProt Comments for AARS2
AARSL: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS2 are the cause of combined oxidative phosphorylation deficiency type 8 (COXPD8). A mitochondrial disease characterized by a lethal infantile hypertrophic cardiomyopathy, generalized muscle dysfunction and some neurologic involvement. The liver is not affected. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Ligase; EC 6.1.1.7; Translation

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: mitochondrion

Molecular Function: zinc ion binding; alanine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: mitochondrial respiratory chain complex assembly; tRNA aminoacylation for protein translation; gene expression

Disease: Leukoencephalopathy, Progressive, With Ovarian Failure; Combined Oxidative Phosphorylation Deficiency 8
Product References and Citations for anti-AARS2 antibody
Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)
Nakayama,M., Genome Res. 12 (11), 1773-1784 (2002)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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