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anti-ABCB5 antibody :: Goat ABCB5 Polyclonal Antibody

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Catalog # MBS421569
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ABCB5, Polyclonal Antibody

Also Known As

Goat anti-ABCB5 Antibody

Product Synonym Names
Goat Anti-ABCB5 (aa364-378) Antibody; ATP-binding cassette, sub-family B (MDR/TAP), member 5; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ATP-binding cassette; sub-family B (MDR/TAP); member 5 antibody; ABCB5alpha antibody; ABCB5beta antibody; EST422562 antibody; ATP-binding cassette; sub-family B; member 5N: P-glycoprotein ABCB5 antibody
Antibody/Peptide Pairs
ABCB5 peptide (MBS428014) is used for blocking the activity of ABCB5 antibody (MBS421569)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
812
Immunogen Sequence
QTQHRNTSKK AQ
OMIM
611785
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QTQHRNTSKKAQ, from the internal region of the protein sequence according to NP_848654.3; NP_001157413.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ABCB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ABCB5 antibody
Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ABCB5 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1ug/ml.
Flow cytometry: This antibody has been successfully used in FC for Hep3B cells as described in the following paper: Cheung et al, Gastroenterology.2011 Jan;140(1): 344-55, PMID: 20682318. Data obtained from a former batch.

Western Blot (WB) of anti-ABCB5 antibody
(0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ABCB5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ABCB5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001157413.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
89,832 Da
NCBI Official Full Name
ATP-binding cassette sub-family B member 5 isoform 2
NCBI Official Synonym Full Names
ATP binding cassette subfamily B member 5
NCBI Official Symbol
ABCB5  [Similar Products]
NCBI Official Synonym Symbols
ABCB5beta; EST422562; ABCB5alpha
  [Similar Products]
NCBI Protein Information
ATP-binding cassette sub-family B member 5
UniProt Protein Name
ATP-binding cassette sub-family B member 5
UniProt Synonym Protein Names
ABCB5 P-gp; P-glycoprotein ABCB5
Protein Family
UniProt Gene Name
ABCB5  [Similar Products]
UniProt Entry Name
ABCB5_HUMAN
NCBI Summary for ABCB5
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]
UniProt Comments for ABCB5
ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; efflux transmembrane transporter activity; xenobiotic-transporting ATPase activity

Biological Process: antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; cell differentiation; compound eye corneal lens development; metabolic process; multidrug transport; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; regulation of membrane potential; transmembrane transport; xenobiotic transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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