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anti-TNK2 antibody :: Rabbit anti-Human Ack1 Polyclonal Antibody

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Catalog # MBS611868
Unit / Price
  0.1 mL  /  $765 +1 FREE 8GB USB
anti-TNK2 antibody
Product Name

Ack1 (TNK2), Polyclonal Antibody

Also Known As

Ack1, phosphorylated (Tyr284) (TNK2, Tyrosine Kinase, non-receptor 2, Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758, FLJ45547, p21cdc42Hs)

Product Synonym Names
Anti -Ack1, phosphorylated (Tyr284) (TNK2, Tyrosine Kinase, non-receptor 2, Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758, FLJ45547, p21cdc42Hs)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 3; NC_000003.11 (195590236..195635880, complement). Location: 3q29
OMIM
606994
3D Structure
ModBase 3D Structure for Q07912
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Recognizes human ACK1 phosphorylated at Tyrosine residue 284 at ~114kD.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, 30% glycerol.
Immunogen
Synthetic peptide corresponding to amino acids surrounding Tyr284 of human ACK1 conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-TNK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TNK2 antibody
Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region. Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility. Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer.
Applications Tested/Suitable for anti-TNK2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-TNK2 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:10,000. Detects ACK1 phosphorylated on Tyrosine residue 284 in COS7 cells transfected with wild type ACK1 but not in COS7 cells transfected with ACK1(Y284F). Lysates from COS7 cells, transfected with either wild type ACK1 or mutant ACK1 (Y284F), were resolved by electrophoresis, transferred to PVDF (Immobilon-P), and probed with A0650-01 for 2 hours at RT. Proteins were visualized via HRP and chemiluminescent detection.
NCBI/Uniprot data below describe general gene information for TNK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
114,569 Da[Similar Products]
NCBI Official Full Name
activated CDC42 kinase 1 isoform 2
NCBI Official Synonym Full Names
tyrosine kinase, non-receptor, 2
NCBI Official Symbol
NCBI Official Synonym Symbols
ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs
  [Similar Products]
NCBI Protein Information
activated CDC42 kinase 1; ACK-1; OTTHUMP00000208563; OTTHUMP00000208564; OTTHUMP00000208565; OTTHUMP00000208566; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; tyrosine kinase non-receptor protein 2
UniProt Protein Name
Activated CDC42 kinase 1
UniProt Synonym Protein Names
Tyrosine kinase non-receptor protein 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ACK1_HUMAN
NCBI Summary for TNK2
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]
UniProt Comments for TNK2
Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.11.1; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasmic vesicle membrane; clathrin-coated vesicle; dendrite; coated pit; extrinsic to internal side of plasma membrane; growth cone; adherens junction; cell soma; membrane; axon; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; WW domain binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; GTPase inhibitor activity; ATP binding; epidermal growth factor receptor binding; hormone receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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