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anti-ACOT8 antibody :: Rabbit anti-Human ACOT8 Polyclonal Antibody

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Catalog # MBS9605192
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

ACOT8, Polyclonal Antibody

Popular Item
Full Product Name

ACOT8 Antibody

Product Synonym Names
ACTEIII; Acot8; ACOT8_HUMAN; Acyl CoA thioesterase 8; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8; Choloyl coenzyme A thioesterase; Choloyl-coenzyme A thioesterase; hACTE III; hACTE-III; hACTEIII; HIV Nef associated acyl CoA thioesterase; HIV-Nef-associated acyl-CoA thioesterase; HNAACTE; hTE; Long chain fatty acyl CoA hydrolase; Palmitoyl CoA hydrolase; Peroxisomal acyl CoA thioesterase 1; Peroxisomal acyl coenzyme A thioester hydrolase 1; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long chain acyl CoA thioesterase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE 1; PTE 2; PTE-1; PTE-2; PTE1; PTE2; Thioesterase II
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O14734
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
ACOT8 antibody detects endogenous levels of total ACOT8
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Peroxisome.
Tissue Specificity
Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (90%), Bovine (90%), Horse (100%), Sheep (90%), Rabbit (90%), Dog (90%), Chicken (90%), Xenopus (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-ACOT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACOT8 antibody
Function: Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233). Involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).
Subunit Structure: Homodimer (By similarity).
Similarity: Belongs to the C/M/P thioester hydrolase family.
Applications Tested/Suitable for anti-ACOT8 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ACOT8 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-ACOT8 antibody
MBS9605192 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-ACOT8 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-ACOT8 antibody
Western blot analysis of extracts from HUVEC cells, using ACOT8 antibody.
anti-ACOT8 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ACOT8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 36 kD
Predicted: 36 kDa
NCBI Official Full Name
acyl-coenzyme A thioesterase 8
NCBI Official Synonym Full Names
acyl-CoA thioesterase 8
NCBI Official Symbol
ACOT8  [Similar Products]
NCBI Official Synonym Symbols
hTE; NAP1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III
  [Similar Products]
NCBI Protein Information
acyl-coenzyme A thioesterase 8
UniProt Protein Name
Acyl-coenzyme A thioesterase 8
UniProt Synonym Protein Names
Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE
UniProt Gene Name
ACOT8  [Similar Products]
UniProt Synonym Gene Names
ACTEIII; PTE1; PTE2; Acyl-CoA thioesterase 8; PTE-1; hACTE-III; hACTEIII; hTE  [Similar Products]
NCBI Summary for ACOT8
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
UniProt Comments for ACOT8
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233). Involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Diseases associated with anti-ACOT8 antibody
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