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anti-ACTN1 antibody :: Rabbit anti-Human Actinin Alpha 1 Polyclonal Antibody

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Catalog # MBS2032441 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Actinin Alpha 1 (ACTN1), Polyclonal Antibody

Full Product Name

Actinin Alpha 1 (ACTN1) Polyclonal Antibody

Product Synonym Names
Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1
Matching Pairs
Matching Pairs
Unconjugated Antibody: Actinin Alpha 1 (MBS2032441)
APC-CY7 Conjugated Antibody: Actinin Alpha 1 (ACTN1) (MBS2064178)
Matching Pairs
Unconjugated Antibody: Actinin Alpha 1 (MBS2032441)
PE Conjugated Antibody: Actinin Alpha 1 (ACTN1) (MBS2064179)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X15804 mRNA
3D Structure
ModBase 3D Structure for P12814
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against ACTN1. It has been selected for its ability to recognize ACTN1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Human
Fragment
ACTN1 (Ala28~Asn260)
Quality Control
Content: The quality control contains recombinant ACTN1 (Ala28~Asn260) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064180
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ACTN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACTN1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-ACTN1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-ACTN1 antibody
Western Blot: Sample: Recombinant protein.
anti-ACTN1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ACTN1 antibody
DAB staining on fromalin fixed paraffin- embedded kidney tissue)
anti-ACTN1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ACTN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
107,141 Da
NCBI Official Full Name
alpha-actinin-1 isoform b
NCBI Official Synonym Full Names
actinin alpha 1
NCBI Official Symbol
ACTN1  [Similar Products]
NCBI Official Synonym Symbols
BDPLT15
  [Similar Products]
NCBI Protein Information
alpha-actinin-1
UniProt Protein Name
Alpha-actinin-1
UniProt Synonym Protein Names
Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1
Protein Family
UniProt Gene Name
ACTN1  [Similar Products]
NCBI Summary for ACTN1
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ACTN1
ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24.1|14q22-q24

Cellular Component: actin filament; brush border; cell projection; cytoplasm; cytosol; extracellular exosome; extracellular region; extracellular space; fascia adherens; focal adhesion; intercellular junction; intracellular; plasma membrane; pseudopodium; ruffle; stress fiber; Z disc

Molecular Function: actin filament binding; calcium ion binding; double-stranded RNA binding; integrin binding; ion channel binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein homodimerization activity; vinculin binding

Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation; actin filament organization; focal adhesion assembly; negative regulation of cell motility; platelet activation; platelet degranulation; platelet formation; regulation of apoptosis

Disease: Bleeding Disorder, Platelet-type, 15
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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