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anti-ATF4 antibody :: Rabbit anti-Human Activating Transcription Factor 4 Polyclonal Antibody

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Catalog # MBS2028259 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $170 +1 FREE 8GB USB
  0.1 mg  /  $220 +1 FREE 8GB USB
  0.2 mg  /  $320 +1 FREE 8GB USB
  1 mg  /  $760 +1 FREE 8GB USB
Product Name

Activating Transcription Factor 4 (ATF4), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Activating Transcription Factor 4 (ATF4)

Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
D90209 mRNA
3D Structure
ModBase 3D Structure for P18848
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against ATF4. It has beenselected for its ability to recognize ATF4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
ATF4 (Thr130~Lys347)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant ATF4 (Thr130~Lys347) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054614
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ATF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ATF4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-ATF4 antibody
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ATF4 antibody
Western Blot: Sample: Recombinant ATF4, Human.
anti-ATF4 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ATF4 antibody
Figur2. DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue
anti-ATF4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ATF4 antibody
DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.
anti-ATF4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ATF4 antibody
DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.
anti-ATF4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ATF4 antibody
DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.
anti-ATF4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ATF4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,590 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-4
NCBI Official Synonym Full Names
activating transcription factor 4
NCBI Official Symbol
NCBI Official Synonym Symbols
CREB2; TXREB; CREB-2; TAXREB67
  [Similar Products]
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-4
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-4
UniProt Synonym Protein Names
Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67
UniProt Gene Name
UniProt Synonym Gene Names
CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67  [Similar Products]
NCBI Summary for ATF4
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
UniProt Comments for ATF4
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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